Back to Search
Start Over
CD5L is a pleiotropic player in liver fibrosis controlling damage, fibrosis and immune cell content
- Source :
- Digital.CSIC: Repositorio Institucional del CSIC, Consejo Superior de Investigaciones Científicas (CSIC), EBioMedicine, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Digital.CSIC. Repositorio Institucional del CSIC
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- © 2019 The Authors.<br />[Background]: Chronic hepatic inflammation leads to liver fibrosis, which may progress to cirrhosis, a condition with high morbidity. Our aim was to assess the as yet unknown role of innate immunity protein CD5L in liver fibrosis. [Methods]: CD5L was measured by ELISA in plasma samples from cirrhotic (n = 63) and hepatitis (n = 39) patients, and healthy controls (n = 7), by immunohistochemistry in cirrhotic tissue (n = 12), and by quantitative RT-PCR in mouse liver cell subsets isolated by cell sorting. Recombinant CD5L (rCD5L) was administered into a murine model of CCl4-induced fibrosis, and damage, fibrosis and hepatic immune cell infiltration, including the LyC6hi (pro-fibrotic)-LyC6low (pro-resolutive) monocyte ratio were determined. Moreover, rCD5L was added into primary human hepatic stellate cells to study transforming growth factor β (TGFβ) activation responses. [Findings]: Cirrhotic patients showed elevated plasma CD5L concentrations as compared to patients with hepatitis and healthy controls (Mann-Whitney test p<br />This work was supported by grants from the Fundació la Marató de TV3 (MTV3 2013-3610), AGAUR (2016 PROD 00094, 2017-SGR-490) to MRS, CSIC (PIE-201720E092) and also from the Instituto de Salud Carlos III (ISCIII), and ERDFs from the EU, ‘Una manera de hacer Europa’, (PI10/01565, PI13/1906, PI16/0974, PI13/02340, PI09/00751 and PI17/00673 to MRS, CA, and PS-B, respectively and PI14/00703 to LK). MRS, PS-B, CA, and CB were supported by the Miguel Servet (CPII14/00021; CPII15/00041), Ramón y Cajal (RYC-2010-07249), and Juan de la Cierva (FJCI-2014-20,505) programs, respectively.
- Subjects :
- 0301 basic medicine
TGFB
Cirrhosis
Macrophage
Inflammation
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Immune system
SMAD7
Fibrosis
Hepatic stellate cells
medicine
Hepatitis
business.industry
Apoptosis inhibitor of macrophages
General Medicine
medicine.disease
030104 developmental biology
030220 oncology & carcinogenesis
Hepatocellular carcinoma
Immunology
Hepatic stellate cell
medicine.symptom
business
Transforming growth factor
Subjects
Details
- ISSN :
- 23523964
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC: Repositorio Institucional del CSIC, Consejo Superior de Investigaciones Científicas (CSIC), EBioMedicine, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Digital.CSIC. Repositorio Institucional del CSIC
- Accession number :
- edsair.doi.dedup.....3dc1dea03e6bf58ba106cf6889a9746c
- Full Text :
- https://doi.org/10.13039/100008666