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ASIC2 Synergizes with TRPV1 in the Mechano-Electrical Transduction of Arterial Baroreceptors
- Source :
- Neurosci Bull
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Mechanosensitive ion channels (MSCs) are key molecules in the mechano-electrical transduction of arterial baroreceptors. Among them, acid-sensing ion channel 2 (ASIC2) and transient receptor potential vanilloid subfamily member 1 (TRPV1) have been studied extensively and documented to play important roles. In this study, experiments using aortic arch–aortic nerve preparations isolated from rats revealed that both ASIC2 and TRPV1 are functionally necessary, as blocking either abrogated nearly all pressure-dependent neural discharge. However, whether ASIC2 and TRPV1 work in coordination remained unclear. So we carried out cell-attached patch-clamp recordings in HEK293T cells co-expressing ASIC2 and TRPV1 and found that inhibition of ASIC2 completely blocked stretch-activated currents while inhibition of TRPV1 only partially blocked these currents. Immunofluorescence staining of aortic arch–aortic adventitia from rats showed that ASIC2 and TRPV1 are co-localized in the aortic nerve endings, and co-immunoprecipitation assays confirmed that the two proteins form a compact complex in HEK293T cells and in baroreceptors. Moreover, protein modeling analysis, exogenous co-immunoprecipitation assays, and biotin pull-down assays indicated that ASIC2 and TRPV1 interact directly. In summary, our research suggests that ASIC2 and TRPV1 form a compact complex and function synergistically in the mechano-electrical transduction of arterial baroreceptors. The model of synergism between MSCs may have important biological significance beyond ASIC2 and TRPV1. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12264-021-00737-1.
- Subjects :
- Baroreceptor
Physiology
TRPV1
TRPV Cation Channels
Pressoreceptors
Transient receptor potential channel
Adventitia
medicine
Animals
Humans
Ion channel
Chemistry
musculoskeletal, neural, and ocular physiology
General Neuroscience
General Medicine
Rats
Cell biology
Acid Sensing Ion Channels
HEK293 Cells
medicine.anatomical_structure
nervous system
cardiovascular system
Original Article
lipids (amino acids, peptides, and proteins)
Mechanosensitive channels
Transduction (physiology)
Free nerve ending
Subjects
Details
- ISSN :
- 19958218 and 16737067
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Neuroscience Bulletin
- Accession number :
- edsair.doi.dedup.....3dc054ce1b1f93e5a4690ff2ea0fa1e0