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Microarray profiling of microRNAs expressed in testis tissues of developing primates
- Source :
- Yan, N, Lu, Y, Sun, H, Qiu, W, Tao, D, Liu, Y, Chen, H, Yang, Y, Zhang, S, Li, X & Ma, Y 2009, ' Microarray profiling of microRNAs expressed in testis tissues of developing primates ', Journal of Assisted Reproduction and Genetics, vol. 26, no. 4, pp. 179-186 . https://doi.org/10.1007/s10815-009-9305-y
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- MicroRNAs (miRNAs) are small non-coding RNA molecules that have been identified as potent regulators of gene expression. Recent studies indicate that miRNAs are involved in mammalian spermatogenesis but the mechanism of regulation is largely unknown.miRNA microarray was employed to compare miRNA expression profiles of testis tissues from immature rhesus monkey (Sample IR), mature rhesus monkey (Sample MR), and mature human (Sample MH). Real-time RT-PCR was used to confirm the changed miRNAs.Twenty-six miRNAs were shared by samples IR/MR and IR/MH with differential expression patterns greater than three-fold difference. PicTar and TargetScan prediction tools predicted a number of target mRNAs, and some of these target genes predicted by miRNAs have been shown to associate with spermatogenesis.Our results indicate that miRNAs are extensively involved in spermatogenesis and provide additional information for further studies of spermatogenetic mechanisms.
- Subjects :
- Adult
Male
Biology
Testis
Gene expression
microRNA
Genetics
Animals
Humans
Spermatogenesis
Genetics (clinical)
Oligonucleotide Array Sequence Analysis
Mechanism (biology)
Obstetrics and Gynecology
RNA
General Medicine
Macaca mulatta
Human genetics
Cell biology
MicroRNAs
Real-time polymerase chain reaction
Reproductive Medicine
Microarray profiling
Developmental Biology
Subjects
Details
- ISSN :
- 15737330 and 10580468
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Assisted Reproduction and Genetics
- Accession number :
- edsair.doi.dedup.....3d9daa80acc724cc242cf28531d1d534