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Control of Cell Identity Genes Occurs in Insulated Neighborhoods in Mammalian Chromosomes
- Source :
- PMC
- Publication Year :
- 2014
- Publisher :
- Elsevier, 2014.
-
Abstract
- The pluripotent state of embryonic stem cells (ESCs) is produced by active transcription of genes that control cell identity and repression of genes encoding lineage-specifying developmental regulators. Here, we use ESC cohesin ChIA-PET data to identify the local chromosomal structures at both active and repressed genes across the genome. The results produce a map of enhancer-promoter interactions and reveal that super-enhancer-driven genes generally occur within chromosome structures that are formed by the looping of two interacting CTCF sites co-occupied by cohesin. These looped structures form insulated neighborhoods whose integrity is important for proper expression of local genes. We also find that repressed genes encoding lineage-specifying developmental regulators occur within insulated neighborhoods. These results provide insights into the relationship between transcriptional control of cell identity genes and control of local chromosome structure.<br />National Institutes of Health (U.S.) (Grant HG002668)
- Subjects :
- Pluripotent Stem Cells
CCCTC-Binding Factor
Chromatin Immunoprecipitation
Chromosomal Proteins, Non-Histone
Cell Cycle Proteins
Biology
Genome
General Biochemistry, Genetics and Molecular Biology
Article
Mice
03 medical and health sciences
0302 clinical medicine
Super-enhancer
Animals
Induced pluripotent stem cell
Gene
Embryonic Stem Cells
030304 developmental biology
Genetics
0303 health sciences
Cohesin
Biochemistry, Genetics and Molecular Biology(all)
High-Throughput Nucleotide Sequencing
Chromosome
Sequence Analysis, DNA
Chromosomes, Mammalian
Embryonic stem cell
Repressor Proteins
Organ Specificity
CTCF
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PMC
- Accession number :
- edsair.doi.dedup.....3d8d7a0d96b3eefac784d1a3805a627b