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HPLC Analysis and Skin Whitening Effects of Umbelliprenin-containing Extracts of Anethum Graveolens, Pimpinella Anisum, and Ferulago Campestris

Authors :
Vito Alessandro Taddeo
Serena Fiorito
Nicolas Caron
Francesca Preziuso
Salvatore Genovese
Francesco Epifano
Philippe de Medina
Arnaud Rives
Marc Poirot
Sandrine Silvente-Poirot
Dipartimento di Scienze del Farmaco
Università degli studi 'G. d'Annunzio' Chieti-Pescara [Chieti-Pescara] (Ud'A)
Affichem S.A. [Toulouse, France] (Société de Biotechnologie médicale)
Centre de Recherches en Cancérologie de Toulouse (CRCT)
Université Toulouse III - Paul Sabatier (UT3)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
This research was in part funded (chemical experiments and assays) by University 'G. d’Annunzio' of Chieti-Pescara, grant number 'Fondi FAR 2017'.
Poirot, Marc
Source :
Molecules, Molecules, MDPI, 2019, 24 (3), pp.501. ⟨10.3390/molecules24030501⟩, Molecules, Vol 24, Iss 3, p 501 (2019), Molecules, 2019, 24 (3), pp.501. ⟨10.3390/molecules24030501⟩
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

Umbelliprenin has recently been shown to have great potential as a skin whitening agent. Wishing to investigate the same effect in plant species known to biosynthesize this coumarin, three plants belonging to the Apiaceae family, namely Anethum graveolens L. (dill), Pimpinella anisum L. (anise), and Ferulago campestris (Besser) Grecescu (field ferula) were screened by HPLC analysis for their respective content of umbelliprenin in extracts obtained with different solvent mixtures and by maceration and ultrasound-assisted processes. EtOH was shown to be the best solvent, providing umbelliprenin yields ranging from 1.7% to 14.4% (with respect to the total amount of extract obtained). Extracts with the highest content of this farnesyloxycoumarin were then assayed as modulators of melanogenesis in cultured murine Melan A cells employing the same umbelliprenin obtained by chemical synthesis as the reference. A parallelism between the content of the coumarin and the recorded depigmenting effect (60% for the EtOH extract of F. campestris as the best value) was revealed for all plants extracts when applied at a dose of 100 μg/mL. Our results demonstrate that the same potential of umbelliprenin can be ascribed also to umbelliprenin-enriched plant extracts which reinforces enforce the widespread use of phyto-preparations for cosmetic purposes (e.g., A. graveolens).

Details

ISSN :
14203049
Volume :
24
Database :
OpenAIRE
Journal :
Molecules
Accession number :
edsair.doi.dedup.....3d7c6077ae30161cae29eba23ebc6820
Full Text :
https://doi.org/10.3390/molecules24030501