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The dichloromethane extract of the ethnomedicinal plant Neurolaena lobata inhibits NPM/ALK expression which is causal for anaplastic large cell lymphomagenesis
- Source :
- International journal of oncology. 42(1)
- Publication Year :
- 2012
-
Abstract
- The present study investigates extracts of Neuolaena lobata, an anti-protozoan ethnomedicinal plant of the Maya, regarding its anti-neoplastic properties. Firstly, extracts of increasing polarity were tested in HL-60 cells analyzing inhibition of cell proliferation and apoptosis induction. Secondly, the most active extract was further tested in anaplastic large cell lymphoma (ALCL) cell lines of human and mouse origin. The dichloromethane extract inhibited proliferation of HL-60, human and mouse ALCL cells with an IC50 of ~2.5, 3.7 and 2.4 µg/ml, respectively and arrested cells in the G2/M phase. The extract induced the checkpoint kinases Chk1 and Chk2 and perturbed the orchestrated expression of the Cdc25 family of cell cycle phosphatases which was paralleled by the activation of p53, p21 and downregulation of c-Myc. Importantly, the expression of NPM/ALK and its effector JunB were drastically decreased, which correlated with the activation of caspase 3. Subsequently also platelet derived growth factor receptor β was downregulated, which was recently shown to be transcriptionally controlled by JunB synergizing with ALK in ALCL development. We show that a traditional healing plant extract downregulates various oncogenes, induces tumor suppressors, inhibits cell proliferation and triggers apoptosis of malignant cells. The discovery of the 'Active Principle(s)' is warranted.
- Subjects :
- Cancer Research
Cdc25
JUNB
Cell
Blotting, Western
Apoptosis
Asteraceae
Immunoenzyme Techniques
Mice
Cell Movement
hemic and lymphatic diseases
medicine
Tumor Cells, Cultured
Animals
Humans
Anaplastic large-cell lymphoma
Cell Proliferation
Methylene Chloride
Plants, Medicinal
biology
Cell growth
Plant Extracts
Cell Cycle
Cell cycle
Protein-Tyrosine Kinases
medicine.disease
medicine.anatomical_structure
Oncology
Cell culture
Immunology
biology.protein
Cancer research
Lymphoma, Large-Cell, Anaplastic
Platelet-derived growth factor receptor
Phytotherapy
Subjects
Details
- ISSN :
- 17912423
- Volume :
- 42
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International journal of oncology
- Accession number :
- edsair.doi.dedup.....3d3846207e0ea0d13f74222dc925215f