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Behaviour of fibroblasts on water born acrylonitrile-based copolymers containing different cationic and anionic moieties
- Source :
- Scharnagl, N.; Hiebl, B.; Trescher, K.; Zierke, M.; Behl, M.; Kratz, K.; Jung, F.; Lendlein, A.: Behaviour of fibroblasts on water born acrylonitrile-based copolymers containing different cationic and anionic moieties. In: Clinical Hemorheology and Microcirculation. Vol. 52 (2012) 2-4, 295-311. (DOI: 10.3233/CH-2012-1606)
- Publication Year :
- 2012
- Publisher :
- IOS Press, 2012.
-
Abstract
- The chemical composition of a substrate can influence the adhesion, viability and proliferation of cells seeded on the substrate. The aim of this work was to investigate the influence of different cationic or anionic moieties in acrylonitrile-based copolymers on the interaction with fibroblasts. A series of ten different types of acrylonitrile-based copolymers with a random sequence structure was prepared using a water born synthesis process to exclude potential residues of organic solvents. As charged comonomers cationic methacrylic acid-2-aminoethylester hydrochloride (AEMA), N-3-amino-propyl-methacrylamide hydrochloride (APMA) and anionic 2-methyl-2-propene-1-sulfonic acid sodium salt (NaMAS) were utilized. By application of a specific sintering procedure the copolymer materials were processed into transparent disks for conducting cell tests in direct contact. The copolymers were analyzed with respect to their composition and surface properties. Cytotoxicity tests of the polymer extracts, as well as of the disks were performed with L929 mouse fibroblasts. All copolymers showed no cytotoxic effects. Furthermore, for higher molar ratios of AEMA an increase in cell growth could be observed, which might be a hint that higher charge densities are favorable for the proliferation of L929 cells.
- Subjects :
- Anions
Male
Cell Survival
Physiology
Hydrochloride
Cell Line
Mice
chemistry.chemical_compound
Polymethacrylic Acids
Cations
Physiology (medical)
Polymer chemistry
Cell Adhesion
Copolymer
Animals
Humans
ddc:610
Chemical composition
chemistry.chemical_classification
Mice, Inbred C3H
Acrylonitrile
Cationic polymerization
Water
Substrate (chemistry)
Hematology
Polymer
Adhesion
Fibroblasts
chemistry
Microscopy, Electron, Scanning
Cardiology and Cardiovascular Medicine
Subjects
Details
- ISSN :
- 13860291
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Clinical Hemorheology and Microcirculation
- Accession number :
- edsair.doi.dedup.....3d27dc30a8e3b33df3b7fc574bf589ad