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The chemical structure and anti-aging bioactivity of an acid polysaccharide obtained from rose buds
- Source :
- Food & Function, Food & Function, 2018, 9 (4), pp.2300--2312. ⟨10.1039/C8FO00206A⟩
- Publication Year :
- 2018
-
Abstract
- An acid polysaccharide, named R-PL, was extracted from rose buds by hot water (80 °C) extraction and purified by Sephadex G-200. R-PL, extracted with a total yield of 1.90%, is a highly pure polysaccharide with a total sugar content of 92.51%. A single and symmetrically sharp peak in its high-performance gel-permeation chromatography (HPGPC) spectrum indicates that R-PL is a homogeneous polysaccharide with a molecular weight of 7.727 × 105 Da. Monosaccharide composition analysis shows that it consists of D-arabinose, D-xylose, D-glucose, D-galactose, D-galacturonic acid, and D-glucuronic acid with a molar ratio of 4.6 : 1.4 : 5.22 : 4.81 : 1 : 1.86. Structure analysis, such as FTIR spectroscopy, periodic acid oxidation, Smith degradation, methylation, and NMR analysis, reveals that R-PL has a backbone of →1)-α-D-Glcp-(6→ with side chains of →1)-β-D-Galp-(4,6→, →1)-β-D-Araf-(5→, →1)-α-D-Xylp-(4→, →1)-α-D-GlcpA-(2→ and →1)-α-D-GalpA-(4→. Its morphological characteristics were identified by Congo red experiments and scanning electron microscopy (SEM) analysis. The results show that R-PL does not have a triple-helical conformation in solution; its shape resembles twisted ribbons. Anti-oxidative and anti-aging analyses show that R-PL has significant antioxidant and anti-aging abilities in vivo.
- Subjects :
- Aging
Magnetic Resonance Spectroscopy
POLE 3
Chemical structure
02 engineering and technology
Flowers
Polysaccharide
Rosa
01 natural sciences
chemistry.chemical_compound
Polysaccharides
Spectroscopy, Fourier Transform Infrared
Side chain
[CHIM]Chemical Sciences
Animals
chemistry.chemical_classification
010405 organic chemistry
Plant Extracts
Diptera
Periodic acid
General Medicine
Nuclear magnetic resonance spectroscopy
021001 nanoscience & nanotechnology
0104 chemical sciences
Congo red
GOBS
chemistry
Sephadex
Yield (chemistry)
Female
0210 nano-technology
Food Science
Nuclear chemistry
Subjects
Details
- ISSN :
- 2042650X
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Foodfunction
- Accession number :
- edsair.doi.dedup.....3d1af5b0a86d018c4a372618d668105f