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Gene expression profile changes and possible molecular subtypes in differentiated-type nonkeratinizing nasopharyngeal carcinoma
- Source :
- International journal of cancer. 128(4)
- Publication Year :
- 2010
-
Abstract
- Nasopharyngeal carcinoma (NPC) is a human malignant tumor with a high incidence and a poor prognosis in Southern China and South-eastern Asia. In this study, we comprehensively analyzed the gene expression profiles in 24 samples of primary differentiated-type nonkeratining NPC (DNK-NPC) tissues, 24 samples of normal nasopharyngeal tissues and 4 DNK-NPC cell lines using cDNA microarray technology and bioinformatics methods. We found expression level of some genes was wildly alerted in the DNK-NPC samples. In addition, our hierarchical clustering analysis revealed 2 distinctive subtypes of gene expression patterns in DNK-NPC tissue samples. The discriminator genes were identified using a signal-to-noise (S(2)N) algorithm by permuting of the data set 10,000 times. To further characterize the clinical relevance of the tumor subtypes, we evaluated a surrogate marker, CCND2, differentially expressed between the 2 tumor subgroups by using immunohistochemistry in an independent set of 137 DNK-NPC samples. CCND2 was highly expressed in the subgroups with "aggressive" features and was associated with T classification (p = 0.006) and clinical stage (p = 0.013). Patients with high level of CCND2 expression had poorer overall survival than those with low level (p = 0.034). Our results suggest that DNK-NPC can be classified into 2 subtypes based on gene expression patterns, which can be used in determining prognosis and treatment of the tumor.
- Subjects :
- Male
Cancer Research
Pathology
medicine.medical_specialty
Cellular differentiation
Pilot Projects
Biology
Immunoenzyme Techniques
Nasopharynx
Gene expression
otorhinolaryngologic diseases
medicine
Biomarkers, Tumor
Cyclin D2
Humans
RNA, Messenger
Gene
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Cancer
Cell Differentiation
Nasopharyngeal Neoplasms
Middle Aged
medicine.disease
Prognosis
Gene expression profiling
stomatognathic diseases
Oncology
Nasopharyngeal carcinoma
Gene chip analysis
Immunohistochemistry
Female
Subjects
Details
- ISSN :
- 10970215
- Volume :
- 128
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International journal of cancer
- Accession number :
- edsair.doi.dedup.....3d166e98a1e6fc0a824ba870617c7a04