Supplemental Figures 1-7 from Identification of Targetable Recurrent MAP3K8 Rearrangements in Melanomas Lacking Known Driver Mutations

Fig S1. Frequent focal amplification of DNA proximal to MAP3K8 breakpoint from TCGA tumors and overall survival. Fig S2. Breakpoint spanning reads and resulting protein translation from additional TCGA melanoma tumors with MAP3K8 rearrangements. Fig S3. Exogenous expression of full length and truncated MAP3K8 decrease sensitivity of BRAF-mutated cells. Fig S4. Identification of additional potential cell line models with MAP3K8 truncating rearrangements. Fig S5. Viability selectively decreased upon MAP3K8 siRNA knockdown in cells harboring truncating MAP3K8 rearrangements. Fig S6. Differential gene expression analysis identifies pathways enriched in MAP3K8-truncated melanomas. Fig S7. Break-apart FISH scoring of melanoma tissue microarray identifies rearranged and amplified MAP3K8.