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Comparative Analysis of Frozen and Acellularized Vascular Xenografts
- Source :
- The International Journal of Artificial Organs. 28:848-852
- Publication Year :
- 2005
- Publisher :
- SAGE Publications, 2005.
-
Abstract
- BackgroundWe implanted frozen and acellularized porcine xenograft vessels as small-diameter arterial grafts in goats and comparatively analyzed the explanted grafts by gross observation and by light microscopy at predetermined periods.Materials and MethodsPorcine carotid arteries were harvested and immediately stored within a tissue preservation solution at −70°C in a freezer designated for frozen xenograft vessels. The acellularized xenograft vessels were prepared with NaCl-SDS solution and stored frozen until use. One pair of porcine xenograft vessels were used to compare the frozen and acellularized grafts in the bilateral carotid arteries in one goat. The grafts were implanted for one, 3, and 6 months in three animals. Periodic ultrasonographic examinations were performed during the observation period. Explanted grafts were analyzed by gross observation, and by light microscopy.ResultsAll animals survived the experimental procedure without specific problems. Ultrasonographic examinations showed excellent patency in all grafts during the observation period. Gross observations revealed nonthrombotic patent smooth lumens. Microscopic examinations of the explanted grafts showed satisfactory cellular reconstruction to the 6-month stage. Although more inflammatory responses were observed in the early phase of implantation of frozen xenografts than of acellularized xenografts, there was no evidence of significant rejection of the frozen xenografts.ConclusionThese findings suggest that porcine vessel xenografts, regardless of them being acellularized or simply frozen xenografts, can be acceptably implanted in goats as a form of small-diameter vascular graft.
- Subjects :
- Pathology
medicine.medical_specialty
Swine
Carotid arteries
Transplantation, Heterologous
Observation period
030232 urology & nephrology
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
030204 cardiovascular system & hematology
Biomaterials
03 medical and health sciences
0302 clinical medicine
Animals
Medicine
Vascular Patency
Cryopreservation
Microscopy
business.industry
Goats
Graft Survival
General Medicine
Anatomy
Arterial grafts
Carotid Arteries
business
Early phase
Vascular graft
Subjects
Details
- ISSN :
- 17246040 and 03913988
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- The International Journal of Artificial Organs
- Accession number :
- edsair.doi.dedup.....3d01adc177bd82854e2a45f4fb5a0f8b