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Compensatory increase in ahpC gene expression and its role in protecting Burkholderia pseudomallei against reactive nitrogen intermediates

Authors :
Suvit Loprasert
Ratiboot Sallabhan
Skorn Mongkolsuk
Wirongrong Whangsuk
Source :
Archives of microbiology. 180(6)
Publication Year :
2003

Abstract

In the human pathogen Burkholderia pseudomallei, katG encodes the antioxidant defense enzyme catalase-peroxidase. Interestingly, a B. pseudomallei mutant, disrupted in katG, is hyperresistant to organic hydroperoxide. This hyperresistance is due to the compensatory expression of the alkyl hydroperoxide reductase gene ( ahpC) and depends on a global regulator OxyR. The KatG-deficient mutant is also highly resistant to reactive nitrogen intermediates (RNI). When overproduced, the B. pseudomallei AhpC protein, protected cells against killing by RNI. The levels of resistance to both organic peroxide and RNI returned to those of the wild-type when the katG mutant was complemented with katG. These studies establish the partially overlapping defensive activities of KatG and AhpC.

Details

ISSN :
03028933
Volume :
180
Issue :
6
Database :
OpenAIRE
Journal :
Archives of microbiology
Accession number :
edsair.doi.dedup.....3cec8f9352f45d68b634950f2bfcd813