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Compensatory increase in ahpC gene expression and its role in protecting Burkholderia pseudomallei against reactive nitrogen intermediates
- Source :
- Archives of microbiology. 180(6)
- Publication Year :
- 2003
-
Abstract
- In the human pathogen Burkholderia pseudomallei, katG encodes the antioxidant defense enzyme catalase-peroxidase. Interestingly, a B. pseudomallei mutant, disrupted in katG, is hyperresistant to organic hydroperoxide. This hyperresistance is due to the compensatory expression of the alkyl hydroperoxide reductase gene ( ahpC) and depends on a global regulator OxyR. The KatG-deficient mutant is also highly resistant to reactive nitrogen intermediates (RNI). When overproduced, the B. pseudomallei AhpC protein, protected cells against killing by RNI. The levels of resistance to both organic peroxide and RNI returned to those of the wild-type when the katG mutant was complemented with katG. These studies establish the partially overlapping defensive activities of KatG and AhpC.
- Subjects :
- Burkholderia pseudomallei
Mutant
Reductase
Biochemistry
Microbiology
Bacterial Proteins
Gene expression
Genetics
RNA, Messenger
Molecular Biology
Catalase-peroxidase
chemistry.chemical_classification
biology
Sodium Nitrite
Genetic Complementation Test
General Medicine
Gene Expression Regulation, Bacterial
Peroxiredoxins
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
biology.organism_classification
Oxidants
Reactive Nitrogen Species
Peroxides
Oxidative Stress
Enzyme
chemistry
Peroxidases
Catalase
Genes, Bacterial
Mutation
biology.protein
bacteria
Bacteria
Subjects
Details
- ISSN :
- 03028933
- Volume :
- 180
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Archives of microbiology
- Accession number :
- edsair.doi.dedup.....3cec8f9352f45d68b634950f2bfcd813