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Early pregnancy affects the expression of toll-like receptor pathway in ovine thymus
- Source :
- Reproductive Biology. 20:547-554
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Toll-like receptors (TLRs) participates in regulation of the maternal immune tolerance during pregnancy, and the thymus is critical for the adaptive immune system. This study hypothesized whether early pregnancy affected the expression of toll-like receptor pathway in the thymus of ewes. In this study, expression of TLRs, tumor necrosis factor receptor associated factor 6 (TRAF6), interleukin 1 receptor associated kinase 1 (IRAK1) and myeloid differentiation primary response gene 88 (MyD88) was detected in maternal thymus during early pregnancy in sheep. Ovine thymuses were collected on day 16 of the estrous cycle, and days 13, 16 and 25 of pregnancy, and expression of TLR members was analyzed by real-time quantitative PCR, western blot and immunohistochemistry analysis. The results revealed that there were decreases in the expression of the mRNA and proteins of TLR2, IRAK1, TRAF6 and MyD88, but increase in TLR5 mRNA and protein. Furthermore, expression of TLR3 and TLR4 proteins peaked at days 13 and 16 of gestation, and MyD88 protein was located in the epithelial reticular cells and thymic corpuscles. In summary, TLR signaling is implicated in regulation of maternal thymic immune, which may be via downregulation of TLR2, IRAK1, TRAF6 and MyD88 during early pregnancy in sheep.
- Subjects :
- 0301 basic medicine
Toll-Like Receptor Pathway
Gestational Age
Thymus Gland
Biology
Immune tolerance
Andrology
03 medical and health sciences
0302 clinical medicine
Endocrinology
Downregulation and upregulation
Pregnancy
Immune Tolerance
Animals
RNA, Messenger
Receptor
TNF Receptor-Associated Factor 6
Sheep
030219 obstetrics & reproductive medicine
Toll-Like Receptors
Acquired immune system
Toll-Like Receptor 2
Toll-Like Receptor 5
TLR2
Interleukin-1 Receptor-Associated Kinases
030104 developmental biology
Gene Expression Regulation
Myeloid Differentiation Factor 88
TLR3
TLR4
Female
Animal Science and Zoology
Signal Transduction
Developmental Biology
Subjects
Details
- ISSN :
- 1642431X
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Reproductive Biology
- Accession number :
- edsair.doi.dedup.....3cd6ace7639125247be9921e60034788
- Full Text :
- https://doi.org/10.1016/j.repbio.2020.10.003