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Abnormal Sensitivity to Glucose of Human Islets Cultured in a High Glucose Medium: Partial Reversibility after an Additional Culture in a Normal Glucose Medium
- Source :
- Scopus-Elsevier
- Publication Year :
- 1991
- Publisher :
- The Endocrine Society, 1991.
-
Abstract
- In the experimental animal chronic hyperglycemia alters the islet's sensitivity to glucose. In the present study the glucose sensitivity of human pancreatic islets, isolated and purified, obtained from seven human pancreases using an automated method was evaluated. After a 12-h stabilization period, islets were cultured for 48 h in normal (5.5 mmol/L) or high glucose (16.7 mmol/L) medium. Islets were then perifused to study their insulin response to glucose. Islets cultured in the high glucose medium lost glucose-induced insulin release and, when challenged with an acute fall of glucose concentration in the perifusate, showed a paradoxical insulin release. Insulin release in response to 10 mmol/L L-arginine was preserved in these islets, suggesting a selective reduction of the insulin response to glucose. An additional 48-h culture in 5.5 mmol/L glucose medium partially restored the sensitivity to glucose of the previously unresponsive islets. These findings indicate that short term exposure to high glucose concentrations induces a selective glucose insensitivity of human islets, which can be partially reversed by an additional culture in normal glucose medium.
- Subjects :
- endocrine system
medicine.medical_specialty
Time Factors
endocrine system diseases
Arginine
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Biology
Biochemistry
Islets of Langerhans
chemistry.chemical_compound
Endocrinology
Culture Techniques
Internal medicine
Insulin Secretion
medicine
Humans
Insulin
Pancreatic hormone
geography
geography.geographical_feature_category
Pancreatic islets
Biochemistry (medical)
Islet
Culture Media
Kinetics
Glucose
medicine.anatomical_structure
L-Glucose
chemistry
Cell culture
Blood sugar regulation
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....3cd5f8d5f26d5c608c70ffa90c3af1e1