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Exploitation of the diverse insertion sequence element content of dairy Lactobacillus helveticus starters as a rapid method to identify different strains
- Source :
- Journal of microbiological methods. 79(1)
- Publication Year :
- 2009
-
Abstract
- The species Lactobacillus helveticus is a commonly used thermophilic starter and/or adjunct culture for Swiss and Cheddar cheese manufacture. Its use is normally associated with flavour improvement which is known to be associated with culture traits such as rapid autolysis and high proteolytic activity. The genome of the commercial strain, DPC4571, was recently sequenced and found to have an abundance of IS sequences in terms of both abundance (213 intact) and diversity (21 types). Given this unique diversity for a lactic acid bacterium, we investigated whether PCR-based IS fingerprinting could be used as a discriminatory tool to distinguish between different strains of Lb. helveticus . A set of ten primers targeting five of the most numerous groups (ISL 1201 , ISLhe 65 , ISLhe 2 , ISLhe 15 and ISL 2 ) of IS elements was designed. Multiplex-PCR with all primers resulted in 1 – 12 discreet amplicons for each strain tested. The resultant fingerprints (in the 0.5 kb – 3 kb range) were found to be strain specific and reproducible. This approach thus provides a valuable method to distinguish between Lb. helveticus strains while giving some indication of the relative abundance of IS sequences in each strain.
- Subjects :
- Microbiology (medical)
Genetics
DNA, Bacterial
Autolysis (biology)
Lactobacillus helveticus
biology
food and beverages
Reproducibility of Results
Amplicon
biology.organism_classification
Microbiology
Genome
DNA Fingerprinting
law.invention
Bacterial Typing Techniques
law
Multiplex polymerase chain reaction
Swiss cheese
DNA Transposable Elements
Food Microbiology
Insertion sequence
Molecular Biology
Polymerase chain reaction
Subjects
Details
- ISSN :
- 18728359
- Volume :
- 79
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of microbiological methods
- Accession number :
- edsair.doi.dedup.....3cd58a826bf06a69ac883f81229edd1e