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DR*W201/P65 Tetramer Visualization of Epitope-Specific CD4 T-Cell during M. tuberculosis Infection and Its Resting Memory Pool after BCG Vaccination
- Source :
- PLoS ONE, PLoS ONE, Vol 4, Iss 9, p e6905 (2009)
- Publication Year :
- 2009
- Publisher :
- Public Library of Science, 2009.
-
Abstract
- Background In vivo kinetics and frequencies of epitope-specific CD4 T cells in lymphoid compartments during M. tuberculosis infection and their resting memory pool after BCG vaccination remain unknown. Methodology/findings Macaque DR*W201 tetramer loaded with Ag85B peptide 65 was developed to directly measure epitope-specific CD4 T cells in blood and tissues form macaques after M. tuberculosis infection or BCG vaccination via direct staining and tetramer-enriched approach. The tetramer-based enrichment approach showed that P65 epitope-specific CD4 T cells emerged at mean frequencies of approximately 500 and approximately 4500 per 10(7) PBL at days 28 and 42, respectively, and at day 63 increased further to approximately 22,000/10(7) PBL after M. tuberculosis infection. Direct tetramer staining showed that the tetramer-bound P65-specific T cells constituted about 0.2-0.3% of CD4 T cells in PBL, lymph nodes, spleens, and lungs at day 63 post-infection. 10-fold expansion of these tetramer-bound epitope-specific CD4 T cells was seen after the P65 peptide stimulation of PBL and tissue lymphocytes. The tetramer-based enrichment approach detected BCG-elicited resting memory P65-specific CD4 T cells at a mean frequency of 2,700 per 10(7) PBL. Significance Our work represents the first elucidation of in vivo kinetics and frequencies for tetramer-bound epitope-specific CD4 T cells in the blood, lymphoid tissues and lungs over times after M. tuberculosis infection, and BCG immunization.
- Subjects :
- CD4-Positive T-Lymphocytes
Tuberculosis
lcsh:Medicine
Epitopes, T-Lymphocyte
Biology
Epitope
Flow cytometry
Mycobacterium tuberculosis
03 medical and health sciences
Epitopes
0302 clinical medicine
Immunology/Immunity to Infections
medicine
Animals
lcsh:Science
Tuberculosis, Pulmonary
Alleles
030304 developmental biology
Cell Proliferation
0303 health sciences
Multidisciplinary
medicine.diagnostic_test
lcsh:R
Microbiology/Medical Microbiology
HLA-DR Antigens
biology.organism_classification
medicine.disease
Virology
Macaca mulatta
3. Good health
Staining
Microbiology/Immunity to Infections
Vaccination
Kinetics
Immunology
Immunology/Immune Response
BCG Vaccine
lcsh:Q
Lymph
Peptides
BCG vaccine
Immunologic Memory
030215 immunology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 4
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....3cc1ff4b0610d327112863b367061c93