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The mannose cap of mycobacterial lipoarabinomannan does not dominate the Mycobacterium-host interaction
- Source :
- Cellular Microbiology 10 (2008) 4, Appelmelk, B J, den Dunnen, J T, Driessen, N N, Ummels, R, Pak, M, Nigou, J, Larrouy-Maumus, G, Gurcha, S S, Movahedzadeh, F, Geurtsen, J, Brown, E J, Eysink Smeets, M M, Besra, G S, Willemsen, P T, Lowary, T L, van Kooijk, Y, Maaskant, J J, Stoker, N G, van der Ley, P, Puzo, G, Vandenbroucke-Grauls, C M J E, Wieland, C W, van der Poll, T, Geijtenbeek, T B H, van der Sar, A M & Bitter, W 2008, ' The mannose cap of mycobacterial lipoarabinomannan does not dominate the Mycobacterium-host interaction ', Cellular Microbiology, vol. 10, no. 4, pp. 930-944 . https://doi.org/10.1111/j.1462-5822.2007.01097.x, Cellular Microbiology, 10(4), 930-944. Wiley-Blackwell, Cellular microbiology, 10(4), 930-944. Wiley-Blackwell, Appelmelk, B J, den Dunnen, J T, Driessen, N N, Ummels, R, Pak, M, Nigou, J, Larrouy-Maumus, G, Gurcha, S S, Movahedzadeh, F, Geurtsen, J, Brown, E J, Eysink Smeets, M M, Besra, G S, Willemsen, P T, Lowary, T L, van Kooyk, Y, Maaskant, J J, Stoker, N G, van der Ley, P, Puzo, G, Vandenbroucke-Grauls, C M J E, Wieland, C W, van der Poll, T, Geijtenbeek, T B H, van der Sar, A M & Bitter, W 2008, ' The mannose cap of mycobacterial lipoarabinomannan does not dominate the Mycobacterium-host interaction ', Cellular Microbiology, vol. 10, no. 4, pp. 930-944 . https://doi.org/10.1111/j.1462-5822.2007.01097.x, Cellular Microbiology, 10(4), 930-944
- Publication Year :
- 2008
-
Abstract
- Pathogenic mycobacteria have the ability to persist in phagocytic cells and to suppress the immune system. The glycolipid lipoarabinomannan (LAM), in particular its mannose cap, has been shown to inhibit phagolysosome fusion and to induce immunosuppressive IL-10 production via interaction with the mannose receptor or DC-SIGN. Hence, the current paradigm is that the mannose cap of LAM is a crucial factor in mycobacterial virulence. However, the above studies were performed with purified LAM, never with live bacteria. Here we evaluate the biological properties of capless mutants of Mycobacterium marinum and M.bovis BCG, made by inactivating homologues of Rv1635c. We show that its gene product is an undecaprenyl phosphomannose-dependent mannosyltransferase. Compared with parent strain, capless M.marinum induced slightly less uptake by and slightly more phagolysosome fusion in infected macrophages but this did not lead to decreased survival of the bacteria in vitro, nor in vivo in zebra fish. Loss of caps in M.bovis BCG resulted in a sometimes decreased binding to human dendritic cells or DC-SIGN-transfected Raji cells, but no differences in IL-10 induction were observed. In mice, capless M.bovis BCG did not survive less well in lung, spleen or liver and induced a similar cytokine profile. Our data contradict the current paradigm and demonstrate that mannose-capped LAM does not dominate the Mycobacterium-host interaction. © 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd.
- Subjects :
- Lipopolysaccharides
Models, Molecular
binding
receptor
arabinan biosynthesis
Mannose
galactofuranosyltransferase
Mannosyltransferases
chemistry.chemical_compound
Mice
Zebrafish
biology
Interleukin-10
DC-SIGN
mannosyltransferases
tuberculosis
Host-Pathogen Interactions
Electrophoresis, Polyacrylamide Gel
Female
Mannose receptor
Mannosyltransferase
dc-sign
intracellular survival
Immunology
Immunoblotting
Microbiology
Phagolysosome
Models, Biological
Mycobacterium
Virology
Animals
Humans
SDG 14 - Life Below Water
Mycobacterium marinum
Bacterial Capsules
Mycobacterium Infections
Lipoarabinomannan
Macrophages
Genetic Complementation Test
transferase-activity
Dendritic Cells
biology.organism_classification
bacterial infections and mycoses
Mice, Inbred C57BL
Mutagenesis, Insertional
chemistry
Mutation
biology.protein
DNA Transposable Elements
CVI - Divisie Bacteriologie en TSE's
human dendritic cells
Subjects
Details
- ISSN :
- 14625814
- Volume :
- 10
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cellular Microbiology
- Accession number :
- edsair.doi.dedup.....3ca7040c4c9ecff6bd8c8a01cfdb29b1