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Caffeine Positively Modulates Ferritin Heavy Chain Expression in H460 Cells: Effects on Cell Proliferation
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 9, p e0163078 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- Both the methylxanthine caffeine and the heavy subunit of ferritin molecule (FHC) are able to control the proliferation rate of several cancer cell lines. While caffeine acts exclusively as a negative modulator of cell proliferation, FHC might reduce or enhance cell viability depending upon the different cell type. In this work we have demonstrated that physiological concentrations of caffeine reduce the proliferation rate of H460 cells: along with the modulation of p53, pAKT and Cyclin D1, caffeine also determines a significant FHC up-regulation through the activation of its transcriptional efficiency. FHC plays a central role in the molecular pathways modulated by caffeine, ending in a reduced cell growth, since its specific silencing by siRNA almost completely abolishes caffeine effects on H460 cell proliferation. These results allow the inclusion of ferritin heavy subunits among the multiple molecular targets of caffeine and open the way for studying the relationship between caffeine and intracellular iron metabolism.
- Subjects :
- 0301 basic medicine
Protein Extraction
lcsh:Medicine
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Enzyme assays
Small interfering RNAs
Colorimetric assays
lcsh:Science
Bioassays and physiological analysis
Extraction Techniques
Multidisciplinary
MTT assay
Cell biology
Nucleic acids
Chemistry
Cell Processes
030220 oncology & carcinogenesis
Physical Sciences
Caffeine
Intracellular
Research Article
Cell type
Biology
Research and Analysis Methods
Cell Growth
03 medical and health sciences
Cyclin D1
Alkaloids
Genetics
Gene silencing
Viability assay
cardiovascular diseases
Non-coding RNA
Cell Proliferation
Ferritin
Cell growth
lcsh:R
Chemical Compounds
Biology and Life Sciences
Proteins
Protein Complexes
Cell Biology
RNA extraction
Gene regulation
030104 developmental biology
chemistry
Biochemical analysis
biology.protein
RNA
lcsh:Q
Gene expression
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....3c7e601a320910bb4216a475fcfd439e