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Fibroblast transition to an endothelial 'trans' state improves cell reprogramming efficiency
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Fibroblast reprogramming offers the potential for myocardial regeneration via in situ cell transdifferentiation. We explored a novel strategy leveraging endothelial cell plasticity to enhance reprogramming efficiency. Rat cardiac endothelial cells and fibroblasts were treated with Gata4, Mef2c, and Tbx5 (GMT) to assess the cardio-differentiation potential of these cells. The endothelial cell transdifferentiation factor ETV2 was transiently over-expressed in fibroblasts followed by GMT treatment to assess “trans-endothelial” cardio-differentiation. Endothelial cells treated with GMT generated more cTnT+ cells than did cardiac fibroblasts (13% ± 2% vs 4% ± 0.5%, p
- Subjects :
- Cell biology
Science
Cell Plasticity
Cell
Cardiology
Cell Separation
complex mixtures
Article
Andrology
Contractility
Prevalence
medicine
Animals
Humans
Myocytes, Cardiac
Endothelium
Fibroblast
Multidisciplinary
business.industry
Regeneration (biology)
Transdifferentiation
Endothelial Cells
Fibroblasts
Cellular Reprogramming
Flow Cytometry
Coculture Techniques
Rats
Endothelial stem cell
medicine.anatomical_structure
Animals, Newborn
Cell Transdifferentiation
Medicine
business
Reprogramming
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....3c66375873e80fde2b8b80a6c406e66a