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Mechanisms of Iron Uptake from Ferric Phosphate Nanoparticles in Human Intestinal Caco-2 Cells
- Source :
- Nutrients, Vol 9, Iss 4, p 359 (2017), Nutrients, 9 (4), Perfecto, A, Elgy, C, Valsami-Jones, E, Sharp, P, Hilty, F & Fairweather-Tait, S 2017, ' Mechanisms of iron uptake from ferric phosphate nanoparticles in human intestinal Caco-2 cells ', Nutrients, vol. 9, no. 4, 359 . https://doi.org/10.3390/nu9040359, Nutrients
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- Food fortification programs to reduce iron deficiency anemia require bioavailable forms of iron that do not cause adverse organoleptic effects. Rodent studies show that nano-sized ferric phosphate (NP-FePO4) is as bioavailable as ferrous sulfate, but there is controversy over the mechanism of absorption. We undertook in vitro studies to examine this using a Caco-2 cell model and simulated gastrointestinal (GI) digestion. Supernatant iron concentrations increased inversely with pH, and iron uptake into Caco-2 cells was 2–3 fold higher when NP-FePO4 was digested at pH 1 compared to pH 2. The size and distribution of NP-FePO4 particles during GI digestion was examined using transmission electron microscopy. The d50 of the particle distribution was 413 nm. Using disc centrifugal sedimentation, a high degree of agglomeration in NP-FePO4 following simulated GI digestion was observed, with only 20% of the particles ≤1000 nm. In Caco-2 cells, divalent metal transporter-1 (DMT1) and endocytosis inhibitors demonstrated that NP-FePO4 was mainly absorbed via DMT1. Small particles may be absorbed by clathrin-mediated endocytosis and micropinocytosis. These findings should be considered when assessing the potential of iron nanoparticles for food fortification.<br />Nutrients, 9 (4)<br />ISSN:2072-6643
- Subjects :
- Nano iron
Bioavailability
Iron
NP-FePO4
Metal Nanoparticles
lcsh:TX341-641
Ferric Compounds
Article
Caco-2 cells
DMT1
Endocytosis
Simulated gastrointestinal digestion
simulated gastrointestinal digestion
Humans
endocytosis
Particle Size
RNA, Small Interfering
Cation Transport Proteins
digestive, oral, and skin physiology
Epithelial Cells
Hydrogen-Ion Concentration
Intestines
nano iron
Digestion
bioavailability
lcsh:Nutrition. Foods and food supply
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....3c5ad262bc3065960f3373f194e930d7