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Whole-genome expression analysis in primary human keratinocyte cell cultures exposed to 60 GHz radiation

Authors :
Marc Aubry
Fabienne Desmots
Ronan Sauleau
Catherine Le Quément
Denis Michel
Yves Le Dréan
Christophe Nicolas Nicolaz
Maxim Zhadobov
Interactions cellulaires et moléculaires (ICM)
Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)
Institut d'Électronique et des Technologies du numéRique (IETR)
Nantes Université (NU)-Université de Rennes 1 (UR1)
Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes)
Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)
Plateforme Génomique Santé Biogenouest®
Université de Nantes (UN)-Université de Rennes 1 (UR1)
De Villemeur, Hervé
Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)
Université de Nantes (UN)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes)
Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)
Source :
Bioelectromagnetics, Bioelectromagnetics, Wiley, 2012, 33 (2), pp.147-158. ⟨10.1002/bem.20693⟩, Bioelectromagnetics, 2012, 33 (2), pp.147-158. ⟨10.1002/bem.20693⟩
Publication Year :
2011
Publisher :
Wiley, 2011.

Abstract

International audience; The main purpose of this study is to investigate potential responses of skin cells to millimeter wave (MMW) radiation increasingly used in the wireless technologies. Primary human skin cells were exposed for 1, 6, or 24 h to 60.4 GHz with an average incident power density of 1.8 mW/cm(2) and an average specific absorption rate of 42.4 W/kg. A large-scale analysis was performed to determine whether these exposures could affect the gene expression. Gene expression microarrays containing over 41,000 unique human transcript probe sets were used, and data obtained for sham and exposed cells were compared. No significant difference in gene expression was observed when gene expression values were subjected to a stringent statistical analysis such as the Benjamini-Hochberg procedure. However, when a t-test was employed to analyze microarray data, 130 transcripts were found to be potentially modulated after exposure. To further quantitatively analyze these preselected transcripts, real-time PCR was performed on 24 genes with the best combination of high fold change and low P-value. Five of them, namely CRIP2, PLXND1, PTX3, SERPINF1, and TRPV2, were confirmed as differentially expressed after 6 h of exposure. To the best of our knowledge, this is the first large-scale study reporting on potential gene expression modification associated with MMW radiation used in wireless communication applications. Bioelectromagnetics. © 2011 Wiley-Liss, Inc.

Details

ISSN :
01978462 and 1521186X
Volume :
33
Database :
OpenAIRE
Journal :
Bioelectromagnetics
Accession number :
edsair.doi.dedup.....3c36a5ff7e6c1f1b1bb50213a8a15f8d