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Thyroid hormone and vitamin D regulate VGF expression and promoter activity
- Source :
- Journal of Molecular Endocrinology
- Publication Year :
- 2016
- Publisher :
- BioScientifica, 2016.
-
Abstract
- The Siberian hamster (Phodopus sungorus) survives winter by decreasing food intake and catabolizing abdominal fat reserves, resulting in a sustained, profound loss of body weight. Hypothalamic tanycytes are pivotal for this process. In these cells, short-winter photoperiods upregulate deiodinase 3, an enzyme that regulates thyroid hormone availability, and downregulate genes encoding components of retinoic acid (RA) uptake and signaling. The aim of the current studies was to identify mechanisms by which seasonal changes in thyroid hormone and RA signaling from tanycytes might ultimately regulate appetite and energy expenditure. proVGF is one of the most abundant peptides in the mammalian brain, and studies have suggested a role for VGF-derived peptides in the photoperiodic regulation of body weight in the Siberian hamster. In silico studies identified possible thyroid and vitamin D response elements in the VGF promoter. Using the human neuroblastoma SH-SY5Y cell line, we demonstrate that RA increases endogenous VGF expression (PP3 increased endogenous VGF mRNA expression (PP3) decreased both (PP3 blocked the short day-induced increase in VGF expression in the dorsomedial posterior arcuate nucleus of Siberian hamsters. Thus, we conclude that VGF expression is a likely target of photoperiod-induced changes in tanycyte-derived signals and is potentially a regulator of seasonal changes in appetite and energy expenditure.
- Subjects :
- 0301 basic medicine
Male
Transcriptional Activation
medicine.medical_specialty
endocrine system
Phodopus
Cellular differentiation
Deiodinase
Retinoic acid
Hamster
Gene Expression
vitamin D
SH-SY5Y cells
03 medical and health sciences
chemistry.chemical_compound
Endocrinology
Calcitriol
Internal medicine
Cell Line, Tumor
Cricetinae
medicine
Animals
Humans
Nerve Growth Factors
Promoter Regions, Genetic
Molecular Biology
Cell Proliferation
Triiodothyronine
biology
VGF (non-acronymic)
Research
Thyroid
Cell Differentiation
biology.organism_classification
thyroid hormone
Siberian hamster
030104 developmental biology
medicine.anatomical_structure
VGF (non-acronymic), thyroid hormone, SH-SY5Y cells, Siberian hamster, vitamin
chemistry
biology.protein
Hormone
Subjects
Details
- Language :
- English
- ISSN :
- 09525041 and 14796813
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Endocrinology
- Accession number :
- edsair.doi.dedup.....3c2fd129be7b26f025ee06d0ccd02dca