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Subcellular distribution of rat liver NADPH-2,4-dienoyl-CoA reductase
- Source :
- Biologicalpharmaceutical bulletin. 19(2)
- Publication Year :
- 1996
-
Abstract
- The subcellular distribution of 2,4-dienoyl-CoA reductase (EC. 1.3.1.34, DCR) in rat liver was studied biochemically and immunochemically after the induction of clofibrate. DCR activity was mainly detected in the mitochondrial fraction by sucrose density gradient centrifugation in the livers of both normal and clofibrate-treated rats. It was also shown that the polyclonal antibody against purified DCR detected the enzyme in the mitochondrial fraction. However, the antibodies, which were affinity purified using the purified mitochondrial DCR or were epitope-selected using the fusion-polypeptide expressed from the mitochondrial cDNA clone (lambda gt11-RDR181), were cross-reacted with the peroxisomal DCR. These results suggest that peroxisomal DCR is immunochemically indistinguishable from mitochondrial DCR.
- Subjects :
- Fatty Acid Desaturases
Male
Oxidoreductases Acting on CH-CH Group Donors
Blotting, Western
Pharmaceutical Science
2,4 Dienoyl-CoA reductase
Mitochondria, Liver
Reductase
Mitochondrion
Microbodies
medicine
Animals
Rats, Wistar
Unsaturated fatty acid
Pharmacology
chemistry.chemical_classification
Clofibrate
biology
General Medicine
Peroxisome
Molecular biology
Precipitin Tests
eye diseases
Rats
Enzyme
Biochemistry
chemistry
Liver
Polyclonal antibodies
biology.protein
sense organs
medicine.drug
Subjects
Details
- ISSN :
- 09186158
- Volume :
- 19
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biologicalpharmaceutical bulletin
- Accession number :
- edsair.doi.dedup.....3c291544a828a9416c6ef03df592a322