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Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro
- Source :
- Calcified Tissue International. 43:46-49
- Publication Year :
- 1988
- Publisher :
- Springer Science and Business Media LLC, 1988.
-
Abstract
- Mandibular first molars from 17-day-old mouse embryos were cultured for 2 and 4 days in alpha-MEM supplemented with 10% newborn bovine serum containing 0.02% ethanol or 0.1, 1.0 or 10 ng/ml 1,25-(OH)2D3. After embedding, every 6th section was stained and mitotic features of inner dental epithelium (IDE) and dental papilla cells (DP) were counted under a light microscope. On the 2nd day, no significant differences were observed in the IDE and DP mitotic indexes among the control, ethanol, and the three vitamin D groups. On the 4th day, the indexes for all groups decreased. But the IDE indexes for all vitamin D groups were significantly different from those for the control (P less than 0.01) and ethanol (P less than 0.05) groups, whereas the DP index was significant (P less than 0.05) only in the 10 ng/ml vitamin D group. The present results suggest that vitamin D3 may have an influence on cell proliferation of IDE and DP in mouse embryonic molars in vitro.
- Subjects :
- Vitamin
medicine.medical_specialty
Ratón
Endocrinology, Diabetes and Metabolism
Biology
Mice
chemistry.chemical_compound
Calcification, Physiologic
Endocrinology
Calcitriol
Internal medicine
medicine
Vitamin D and neurology
Animals
Orthopedics and Sports Medicine
Dental Enamel
Dental papilla
Cell growth
Cell Differentiation
Embryo
Molar
Epithelium
medicine.anatomical_structure
chemistry
Cell culture
Dentin
Odontogenesis
Cell Division
Subjects
Details
- ISSN :
- 14320827 and 0171967X
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Calcified Tissue International
- Accession number :
- edsair.doi.dedup.....3c218abf8ab9b59012450cc1aa5ec660