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A New Method For Rapid Dna Extraction From Artemia (Branchiopoda, Crustacea)

Authors :
Manaffar, R.
Maleki, R.
Zare, S.
Agh, N.
Soltanian, S.
Sehatnia, B.
Sorgeloos, P.
Peter Bossier
Stappen, G.
Source :
Scopus-Elsevier
Publication Year :
2010
Publisher :
Zenodo, 2010.

Abstract

Artemia is one of the most conspicuous invertebrates associated with aquaculture. It can be considered as a model organism, offering numerous advantages for comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is an important step of any molecular experiment, a new and a rapid method of DNA extraction from adult Artemia was described in this study. Besides, the efficiency of this technique was compared with two widely used alternative techniques, namely Chelex® 100 resin and SDS-chloroform methods. Data analysis revealed that the new method is the easiest and the most cost effective method among the other methods which allows a quick and efficient extraction of DNA from the adult animal.<br />{"references":["J. Dhont, P. Sorgeloos, Applications of Artemia. In: Abatzopoulos, T.,\nBeardmore, J., Clegg, J., Sorgeloos, P. (Eds). Artemia: basic and applied\nbiology. 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Details

Language :
English
Database :
OpenAIRE
Journal :
Scopus-Elsevier
Accession number :
edsair.doi.dedup.....3c16cc890ad146c1e9ba1e9327318c40
Full Text :
https://doi.org/10.5281/zenodo.1058810