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Computationally-efficient spatiotemporal correlation analysis super-resolves anomalous diffusion
- Source :
- Optics express. 29(5)
- Publication Year :
- 2021
-
Abstract
- Anomalous diffusion dynamics in confined nanoenvironments govern the macroscale properties and interactions of many biophysical and material systems. Currently, it is difficult to quantitatively link the nanoscale structure of porous media to anomalous diffusion within them. Fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) has been shown to extract nanoscale structure and Brownian diffusion dynamics within gels, liquid crystals, and polymers, but has limitations which hinder its wider application to more diverse, biophysically-relevant datasets. Here, we parallelize the least-squares curve fitting step on a GPU improving computation times by up to a factor of 40, implement anomalous diffusion and two-component Brownian diffusion models, and make fcsSOFI more accessible by packaging it in a user-friendly GUI. We apply fcsSOFI to simulations of the protein fibrinogen diffusing in polyacrylamide of varying matrix densities and super-resolve locations where slower, anomalous diffusion occurs within smaller, confined pores. The improvements to fcsSOFI in speed, scope, and usability will allow for the wider adoption of super-resolution correlation analysis to diverse research topics.
- Subjects :
- Materials science
Anomalous diffusion
Computation
Fluorescence correlation spectroscopy
02 engineering and technology
01 natural sciences
010309 optics
Diffusion
Matrix (mathematics)
Optics
Spatio-Temporal Analysis
Liquid crystal
0103 physical sciences
Statistical physics
Brownian motion
Fluorescent Dyes
business.industry
Quinolinium Compounds
Computational Biology
Fibrinogen
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
Microscopy, Fluorescence
Curve fitting
0210 nano-technology
Porous medium
business
Subjects
Details
- ISSN :
- 10944087
- Volume :
- 29
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Optics express
- Accession number :
- edsair.doi.dedup.....3c139539062b0eccf780dee166f5b022