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Two cinnamate derivatives produce similar alteration in mRNA expression and activity of antioxidant enzymes in rats
- Source :
- Journal of biochemical and molecular toxicology. 17(5)
- Publication Year :
- 2003
-
Abstract
- Cinnamate is a widespread secondary metabolite of phenolic compound synthesized by plants for defensive purposes. The current study was designed to investigate the effect of two structurally related cinnamate derivatives, 4-hydroxycinnamate and 3-(4-hydroxyphenyl)propionic acid (HPP), on the mRNA expression and activity of antioxidant enzymes in high-cholesterol-fed rats. Male rats were fed a 1 g/100 g high-cholesterol diet with supplements of either 4-hydroxycinnamate or HPP (0.135 mmol/100 g diet) for 6 weeks. The plasma paraoxonase activity was found to be higher in the cinnamate-derivative-supplemented groups than in the control group. The erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities, plus glutathione (GSH) level, were all significantly higher in the 4-hydroxycinnamate- and HPP-supplemented groups than in the control group. However, both 4-hydroxycinnamate and HPP supplementation significantly lowered the hepatic activities and mRNA expression of CAT and glutathione peroxidase (GSH-Px) compared to the control group. The hepatic mRNA expression and activity of SOD did not differ between the groups. The hepatic thiobarbituric acid reactive substances (TBARS) level was significantly lowered by the 4-hydroxycinnamate and HPP supplementation. Accordingly, these results indicate that supplementation by 4-hydroxycinnamate and HPP would seem to enhance the antioxidative defense of erythrocyte. Both HPP and 4-hydroxycinnamate would appear to be beneficial in improving the function of antioxidative enzymes on a molecular level in high-cholesterol-fed rats.
- Subjects :
- Male
medicine.medical_specialty
Antioxidant
Erythrocytes
Coumaric Acids
Thiobarbituric acid
Health, Toxicology and Mutagenesis
medicine.medical_treatment
Toxicology
Biochemistry
Thiobarbituric Acid Reactive Substances
Antioxidants
Superoxide dismutase
Cholesterol, Dietary
Rats, Sprague-Dawley
chemistry.chemical_compound
Internal medicine
medicine
TBARS
Animals
RNA, Messenger
Molecular Biology
chemistry.chemical_classification
biology
Phenylpropionates
Aryldialkylphosphatase
Superoxide Dismutase
Glutathione peroxidase
General Medicine
Glutathione
Blotting, Northern
Catalase
Rats
Enzyme
Endocrinology
chemistry
Liver
biology.protein
Molecular Medicine
Diet, Atherogenic
Propionates
Subjects
Details
- ISSN :
- 10956670
- Volume :
- 17
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of biochemical and molecular toxicology
- Accession number :
- edsair.doi.dedup.....3bf1c15a6acf9a66c644b11e58610fce