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Multilayer interactions determine the Golgi localization of GRIP golgins
- Source :
- Traffic (Copenhagen, Denmark). 7(10)
- Publication Year :
- 2006
-
Abstract
- Golgin-97, RanBP2alpha, Imh1p and p230/golgin-245 (GRIP) domain golgins are targeted to the Golgi membrane through their GRIP domains. By analyzing more than 30 mutants of golgin-97 and golgin-245 GRIP domains for their properties of dimerization, interaction with ARF like protein 1 (Arl1)-GTP and Golgi targeting, we found hierarchically organized three-tier interactions governing the Golgi targeting of GRIP domain golgins. GRIP domain self-dimerization is necessary for bivalent interaction with Arl1-GTP. Unexpectedly, however, these two interactions are not sufficient for Golgi targeting, as a third group of residues, including positive-charged arginine between alpha1 and alpha2 and hydrophobic residues C-terminal to the GRIP domain, turn out to be essential. Surface plasmon resonance analysis indicates that GRIP domain interacts directly with membrane lipid, partially through the third group of residues such as W744 of golgin-97. This third tier of interaction with the membrane could be mediated by non-specific hydrophobic and electrostatic forces.
- Subjects :
- Models, Molecular
Saccharomyces cerevisiae Proteins
Protein Conformation
Recombinant Fusion Proteins
Molecular Sequence Data
Vesicular Transport Proteins
Golgi Apparatus
Sequence alignment
Biology
Biochemistry
Autoantigens
symbols.namesake
Protein structure
Structural Biology
Two-Hybrid System Techniques
Genetics
Animals
Humans
Amino Acid Sequence
Surface plasmon resonance
Golgi localization
Molecular Biology
Peptide sequence
Monomeric GTP-Binding Proteins
Golgi membrane
Golgi Matrix Proteins
Membrane Proteins
Golgi Targeting
Cell Biology
Intracellular Membranes
Golgi apparatus
Lipids
Cell biology
Protein Structure, Tertiary
Rats
Nuclear Pore Complex Proteins
Liposomes
symbols
Dimerization
Sequence Alignment
Molecular Chaperones
trans-Golgi Network
Subjects
Details
- ISSN :
- 13989219
- Volume :
- 7
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Traffic (Copenhagen, Denmark)
- Accession number :
- edsair.doi.dedup.....3bdca5f461ba9ed0a04e41f9312401f2