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Fast screening method for molecular recognition of islet amyloid polypeptide from whole blood samples collected from diabetic patients with disposable stochastic sensors obtained by nanolayer, and nanolayer by nanolayer deposition using cold plasma
- Source :
- Analytical and Bioanalytical Chemistry. 412:4135-4141
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Nanolayer and nanolayer by nanolayer deposition of nanofilms of Ag and C using cold plasma in sequences (Ag, Ag-C, Ag-Ag-C), on porous paper, were used to design three disposable stochastic sensors for the assay of amyloid polypeptide from whole blood. The nanofilms were modified with α-cyclodextrin. The test developed using the nanofilm-based disposable stochastic sensors is used for early detection of diabetes. The wider linear concentration range (1.00 × 10−6–1.00 ng mL−1) and the lower limit of quantification (1.00 × 10−6ng mL−1) were obtained using the disposable stochastic sensors based on Ag-C and Ag-Ag-C, while the highest sensitivity (3.19 × 104 s−1/μg mL−1) was recorded using the disposable stochastic sensor based on Ag-Ag-C. The screening methods were fully validated using whole blood samples from confirmed patients, when the recovery of the islet amyloid polypeptide was higher than 98.00%.
- Subjects :
- Paper
Silver
Time Factors
Materials science
Plasma Gases
Amyloid
Early detection
02 engineering and technology
01 natural sciences
Biochemistry
Analytical Chemistry
Molecular recognition
Limit of Detection
Screening method
Humans
Deposition (phase transition)
Whole blood
geography
Chromatography
geography.geographical_feature_category
010401 analytical chemistry
Electrochemical Techniques
Equipment Design
Plasma
021001 nanoscience & nanotechnology
Islet
Islet Amyloid Polypeptide
Nanostructures
0104 chemical sciences
Diabetes Mellitus, Type 1
Diabetes Mellitus, Type 2
0210 nano-technology
Porosity
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 412
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....3bd6b7dd7d80ee51ac68e2ca50cdbdea