Back to Search
Start Over
Biochemical studies of Piper betle L leaf extract on obese treated animal using 1H-NMR-based metabolomic approach of blood serum samples
- Source :
- Journal of Ethnopharmacology. 194:690-697
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Piper betle L. (PB) belongs to the Piperaceae family. The presence of a fairly large quantity of diastase in the betel leaf is deemed to play an important role in starch digestion and calls for the study of weight loss activities and metabolite profile from PB leaf extracts using metabolomics approach to be performed. PB dried leaves were extracted with 70% ethanol and the extracts were subjected to five groups of rats fed with high fat (HF) and standard diet (SD). They were then fed with the extracts in two doses and compared with a negative control group given water only according to the study protocol. The body weights and food intakes were monitored every week. At the end of the study, blood serum of the experimental animal was analysed to determine the biochemical and metabolite changes. PB treated group demonstrated inhibition of body weight gain without showing an effect on the food intake. In serum bioassay, the PB treated group (HF/PB (100mg/kg and 500mg/kg) showed an increased in glucose and cholesterol levels compared to the Standard Diet (SD/WTR) group, a decrease in LDL level and increase in HDL level when compared with High Fat Diet (HF/WTR) group. For metabolite analysis, two separation models were made to determine the metabolite changes via group activities. The best separation of PCA serum in Model 1 and 2 was achieved in principle component 1 and principle component 2. SUS-Plot model showed that HF group was characterized by high-level of glucose, glycine and alanine. Increase in the β-hydroxybutyrate level similar with SD group animals was evident in the HF/PB(500mg/kg) group. This finding suggested that the administration of 500mg/kg PB extracts leads to increase in oxidation process in the body thus maintaining the body weight and without giving an effect on the appetite even though HF was continuously consumed by the animals until the end of the studies and also a reduction in food intake, thus maintaining their body weight although they were continuously consumed HF.
- Subjects :
- Male
0301 basic medicine
Metabolite
media_common.quotation_subject
030209 endocrinology & metabolism
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Blood serum
Weight loss
Drug Discovery
medicine
Animals
Metabolomics
Obesity
Food science
media_common
Pharmacology
biology
Plant Extracts
Cholesterol
Body Weight
Appetite
Piperaceae
biology.organism_classification
medicine.disease
Piper betle
Rats
Plant Leaves
Diastase
030104 developmental biology
Biochemistry
chemistry
biology.protein
medicine.symptom
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....3bd433980d945fc28a4e9f502f4887e2
- Full Text :
- https://doi.org/10.1016/j.jep.2016.10.022