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Evaluation of DNA damage in COPD patients and its correlation with polymorphisms in repair genes
- Source :
- BMC Medical Genetics
- Publication Year :
- 2013
- Publisher :
- BioMed Central, 2013.
-
Abstract
- Background We investigated a potential link between genetic polymorphisms in genes XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr) with the level of DNA damage and repair, accessed by comet and micronucleus test, in 51 COPD patients and 51 controls. Methods Peripheral blood was used to perform the alkaline and neutral comet assay; and genetic polymorphisms by PCR/RFLP. To assess the susceptibility to exogenous DNA damage, the cells were treated with methyl methanesulphonate for 1-h or 3-h. After 3-h treatment the % residual damage was calculated assuming the value of 1-h treatment as 100%. The cytogenetic damage was evaluated by buccal micronucleus cytome assay (BMCyt). Results COPD patients with the risk allele XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) showed higher DNA damage by comet assay. The residual damage was higher for COPD with risk allele in the four genes. In COPD patients was showed negative correlation between BMCyt (binucleated, nuclear bud, condensed chromatin and karyorrhexic cells) with pulmonary function and some variant genotypes. Conclusion Our results suggest a possible association between variant genotypes in XRCC1 (Arg399Gln), OGG1 (Ser326Cys), XRCC3 (Thr241Met), and XRCC4 (Ile401Thr), DNA damage and progression of COPD.
- Subjects :
- Male
DNA Repair
Genotype
DNA damage
DNA repair
Biology
Bioinformatics
Genetic polymorphisms
DNA Glycosylases
XRCC1
Pulmonary Disease, Chronic Obstructive
XRCC3
Genetics
COPD
Humans
Genetics(clinical)
Genetics (clinical)
Alleles
Aged
Polymorphism, Genetic
DNA repair protein XRCC4
Middle Aged
Methyl Methanesulfonate
Comet assay
DNA-Binding Proteins
X-ray Repair Cross Complementing Protein 1
Micronucleus test
Comet Assay
BMCyt
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712350
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- BMC Medical Genetics
- Accession number :
- edsair.doi.dedup.....3bd38c8279bffeefaf1486b1fb18e9a2