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Endophytic Actinobacteria from the Brazilian Medicinal PlantLychnophora ericoides<scp>Mart</scp>. and the Biological Potential of Their Secondary Metabolites
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Endophytic actinobacteria from the Brazilian medicinal plant Lychnophora ericoides were isolated for the first time, and the biological potential of their secondary metabolites was evaluated. A phylogenic analysis of isolated actinobacteria was accomplished with 16S rRNA gene sequencing, and the predominance of the genus Streptomyces was observed. All strains were cultured on solid rice medium, and ethanol extracts were evaluated with antimicrobial and cytotoxic assays against cancer cell lines. As a result, 92% of the extracts showed a high or moderate activity against at least one pathogenic microbial strain or cancer cell line. Based on the biological and chemical analyses of crude extracts, three endophytic strains were selected for further investigation of their chemical profiles. Sixteen compounds were isolated, and 3-hydroxy-4-methoxybenzamide (9) and 2,3-dihydro-2,2-dimethyl-4(1H)-quinazolinone (15) are reported as natural products for the first time in this study. The biological activity of the pure compounds was also assessed. Compound 15 displayed potent cytotoxic activity against all four tested cancer cell lines. Nocardamine (2) was only moderately active against two cancer cell lines but showed strong activity against Trypanosoma cruzi. Our results show that endophytic actinobacteria from L. ericoides are a promising source of bioactive compounds.
- Subjects :
- 0301 basic medicine
Trypanosoma cruzi
Antiprotozoal Agents
Secondary Metabolism
Bioengineering
Asteraceae
Biochemistry
Actinobacteria
Structure-Activity Relationship
03 medical and health sciences
Parasitic Sensitivity Tests
Cell Line, Tumor
Botany
Humans
Structure–activity relationship
Secondary metabolism
Medicinal plants
Molecular Biology
Cell Proliferation
Biological Products
Plants, Medicinal
Dose-Response Relationship, Drug
Molecular Structure
Traditional medicine
biology
Chemistry
Biological activity
General Chemistry
General Medicine
biology.organism_classification
Antimicrobial
Antineoplastic Agents, Phytogenic
030104 developmental biology
PLANTAS MEDICINAIS
Molecular Medicine
Drug Screening Assays, Antitumor
Brazil
Subjects
Details
- ISSN :
- 16121872
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Chemistry & Biodiversity
- Accession number :
- edsair.doi.dedup.....3bc03d03915e2c513b1cb7809dbbc5dd