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Aptamer-based surface-enhanced resonance Raman scattering assay on a paper fluidic platform for detection of cardiac troponin I
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2020
- Publisher :
- Society of Photo-Optical Instrumentation Engineers, 2020.
-
Abstract
- Significance: Cardiac troponin I (cTnI) is a primary biomarker for diagnosis of myocardial infarction (MI). In contrast to central laboratory tests for cTnI, point-of-care (POC) testing has the advantage of providing results when the patient is first encountered, which helps high-risk patients to be treated more rapidly and low-risk patients to be released in a timely fashion. A paper fluidic platform is good for POC testing because the paper is abundant, low cost, and disposable. However, current cTnI assays on paper platforms use antibodies as the recognition element, which has limitations due to the high cost of production and antibody stability issues at the POC. Aim: To develop an aptamer-based assay on a paper strip using surface-enhanced resonance Raman spectroscopy (SERRS) for detection of cTnI in the clinically relevant range at the POC. Approach: Gold nanoparticles (AuNPs) were functionalized with a Raman reporter molecule, malachite green isothiocyanate. The functionalized AuNPs were encapsulated in a silica shell and provided a SERRS signal using a handheld Raman system with a 638-nm excitation wavelength. A primary aptamer and a secondary aptamer of cTnI were used in a sandwich assay format to bind the cTnI on a test line of a paper fluidic platform. By measuring the SERRS signal from the test line, the concentration of cTnI was quantitatively determined. Results: The aptamer-based SERRS assay on a paper strip had a detection range of 0.016 to 0.1 ng/ml for cTnI, had good selectivity for cTnI compared to three other markers, had good stability over 10 days, and had good performance in the more complex serum sample matrix. Conclusions: The aptamer-based SERRS assay on a paper strip has the potential to provide a sensitive, selective, stable, repeatable, and cost-effective platform for the detection of cTnI toward eventual use in diagnosis of MI at the POC.
- Subjects :
- Paper
Cardiac troponin
aptamer sensor
Aptamer
Resonance Raman spectroscopy
Biomedical Engineering
Metal Nanoparticles
macromolecular substances
Spectrum Analysis, Raman
01 natural sciences
010309 optics
Biomaterials
symbols.namesake
0103 physical sciences
Troponin I
Humans
Fluidics
Chemistry
Surface-enhanced Raman spectroscopy
Atomic and Molecular Physics, and Optics
surface-enhanced Raman spectroscopy
Electronic, Optical and Magnetic Materials
point-of-care testing
myocardial infarction
Colloidal gold
Sensing
symbols
cardiovascular system
Biological Assay
Gold
Raman scattering
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 15602281 and 10833668
- Volume :
- 25
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....3bbde2885c4b8b9b8ad68fcdd13e707c