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Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate
- Source :
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2011, 67 (Pt 11), pp.1457-61. ⟨10.1107/S1744309111037821⟩
- Publication Year :
- 2011
- Publisher :
- International Union of Crystallography, 2011.
-
Abstract
- International audience; An N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues was crystallized both in its apo form and in a binary complex with glucose 6-phosphate. The crystals both belonged to space group P2(1) and diffracted to 2.85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure.
- Subjects :
- MESH: Gene Expression
MESH: Sequence Homology, Amino Acid
Trypanosoma cruzi
MESH: Glucosephosphate Dehydrogenase
Molecular Sequence Data
MESH: Sequence Alignment
Biophysics
Gene Expression
Dehydrogenase
MESH: Amino Acid Sequence
Biology
Glucosephosphate Dehydrogenase
Crystallography, X-Ray
Biochemistry
Substrate Specificity
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
parasitic diseases
Genetics
Glucose-6-phosphate dehydrogenase
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular replacement
Amino Acid Sequence
Peptide sequence
MESH: Crystallization
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
MESH: Humans
MESH: Molecular Sequence Data
Sequence Homology, Amino Acid
030302 biochemistry & molecular biology
Substrate (chemistry)
MESH: Crystallography, X-Ray
Condensed Matter Physics
biology.organism_classification
Crystallography
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Enzyme
chemistry
Crystallization Communications
MESH: Substrate Specificity
Protein quaternary structure
Crystallization
MESH: Trypanosoma cruzi
Sequence Alignment
Subjects
Details
- Language :
- English
- ISSN :
- 17443091
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2011, 67 (Pt 11), pp.1457-61. ⟨10.1107/S1744309111037821⟩
- Accession number :
- edsair.doi.dedup.....3ba9379b7f8b3c864092847dcfa99946