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A novel cancer/testis antigen KP-OVA-52 identified by SEREX in human ovarian cancer is regulated by DNA methylation
- Source :
- International Journal of Oncology
- Publication Year :
- 2012
- Publisher :
- Spandidos Publications, 2012.
-
Abstract
- SEREX has proven to be a powerful method that takes advantage of the presence of spontaneous humoral immune response in some cancer patients. In this study, immunoscreening of normal testis and two ovarian cancer cell line cDNA expression libraries with sera from ovarian cancer patients led to the isolation of 75 independent antigens, designated KP-OVA-1 through KP-OVA-75. Of these, RT-PCR showed KP-OVA-52 to be expressed strongly in normal testis, in ovarian cancer cell lines (3/9) and in ovarian cancer tissues (1/17). The expression of KP-OVA-52 in cancer cells is also induced by the demethylating agent 5‑aza‑2'‑deoxycytidine (ADC). To test immunogenicity, we used the Serum Antibody Detection Assay (SADA) to analyze anti-IgG antibodies against the 75 antigens that were initially isolated by SEREX. Four of the 75 antigens (KP‑OVA‑25, KP‑OVA‑35, KP‑OVA‑68 and KP‑OVA‑73) reacted exclusively with sera from cancer patients. However, KP‑OVA‑52 reacted with 1 of 20 ovarian cancer sera. These data suggest that the KP-OVA-52 can be considered a novel CT antigen that is regulated by DNA methylation.
- Subjects :
- Male
Cancer Research
Antibodies, Neoplasm
Recombinant Fusion Proteins
Decitabine
chemistry.chemical_compound
serologic analysis of recombinant cDNA expression
Testicular Neoplasms
Antigen
Antigens, Neoplasm
Cell Line, Tumor
medicine
Humans
RNA, Messenger
Gene Library
Ovarian Neoplasms
DNA methylation
biology
ovary cancer
Cancer
Articles
respiratory system
medicine.disease
Molecular biology
Demethylating agent
Oncology
chemistry
Cancer cell
Azacitidine
biology.protein
cancer/testis antigen
Cancer/testis antigens
Female
Antibody
Ovarian cancer
Subjects
Details
- ISSN :
- 17912423 and 10196439
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- International Journal of Oncology
- Accession number :
- edsair.doi.dedup.....3ba48390282ba59a51393b91f7d7eb3b