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Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing
- Source :
- Applied and environmental microbiology. 74(20)
- Publication Year :
- 2008
-
Abstract
- Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the 13 C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.
- Subjects :
- clone (Java method)
DNA, Bacterial
Stable-isotope probing
Sulfides
Applied Microbiology and Biotechnology
DNA, Ribosomal
chemistry.chemical_compound
Isotopes
RNA, Ribosomal, 16S
Environmental Microbiology
Benzene
Phylogeny
Ecology
biology
Bacteria
Hybridization probe
Sequence Analysis, DNA
biology.organism_classification
Molecular biology
chemistry
Biochemistry
GenBank
Biodegradation
Restriction fragment length polymorphism
DNA Probes
DNA
Polymorphism, Restriction Fragment Length
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10985336
- Volume :
- 74
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Applied and environmental microbiology
- Accession number :
- edsair.doi.dedup.....3ba25dbe36ce24f61b0073aff8037b39