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Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing

Authors :
Lee J. Kerkhof
Lily Y. Young
Adam C. Mumford
Lora McGuinness
Craig D. Phelps
Amita. R. Oka
Source :
Applied and environmental microbiology. 74(20)
Publication Year :
2008

Abstract

Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the 13 C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.

Details

ISSN :
10985336
Volume :
74
Issue :
20
Database :
OpenAIRE
Journal :
Applied and environmental microbiology
Accession number :
edsair.doi.dedup.....3ba25dbe36ce24f61b0073aff8037b39