Additional file 1 of Near-infrared light reduces β-amyloid-stimulated microglial toxicity and enhances survival of neurons: mechanisms of light therapy for Alzheimer’s disease

Additional file 1: Methods. Figure S1. Flow cytometry gating scheme of dots and histogram-plots of primary microglia cells. Figure S2. Neurons and microglia transferring into a Boyden chamber of two medium-filled compartments separated by a microporous membrane. Figure S3. Approximate molecular weight of Aβ1-42 oligomers following Western blotting under non-denaturing conditions. Results. Figure S4. NIR light activation phagocytosis of Aβ-treated microglia cells. Figure S5. Mitochondrial membrane potential activation following Aβ and 808 nm light treatment. Figure S6. NIR light effect on general ROS generation, mitochondrial ROS generation and ROS generation in the medium measured for Aβ-treated microglia. Figure S7. Increased neuron viability after 808 nm light exposure in co-culture with Aβ stimulated microglia. Figure S8. Increased neuron viability after 808 nm light exposure in co-culture with Aβ-stimulated microglia.