Back to Search Start Over

Production and characterization of monoclonal antibodies to the macrocyclic trichothecene roridin A

Authors :
R. Hack
Erwin Märtlbauer
G. Terplan
Source :
Applied and Environmental Microbiology. 54:2328-2330
Publication Year :
1988
Publisher :
American Society for Microbiology, 1988.

Abstract

Two murine monoclonal antibodies to the macrocyclic trichothecene roridin A are described. Screening for antibody production was performed on absorbed anti-mouse immunoglobulin serum as double-antibody solid phase, and further characterization was done on affinity-purified anti-mouse IgG serum. The antibodies, designated 5G11 and 4H10, had affinity constants for roridin A of 9.25 X 10(7) and 1.7 X 10(7) liters/mol, respectively. In monoclonal antibody-based direct enzyme immunoassays, these IgG1 antibodies had detection limits for roridin A of 0.4 ng/ml (0.02 ng per assay) and 1.8 ng/ml (0.09 ng per assay), respectively. Both antibodies were most specific for the tested macrocyclic trichothecenes. The relative cross-reactivities of antibody 5G11 with roridin A, roridin J, verrucarin A, satratoxin G, and satratoxin H were 100.0, 43.8, 16.7, 3.7, and 18.9%, respectively; for antibody 4H10 they were 100.0, 6.3, 64.0, 4.4, and 4.9%, respectively.

Details

ISSN :
10985336 and 00992240
Volume :
54
Database :
OpenAIRE
Journal :
Applied and Environmental Microbiology
Accession number :
edsair.doi.dedup.....3b92b789666106144c5cb3a7ec91699d
Full Text :
https://doi.org/10.1128/aem.54.9.2328-2330.1988