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Deciphering Transcriptional Regulators of Banana Fruit Ripening by Regulatory Network Analysis
- Source :
- Plant Biotechnology Journal
- Publication Year :
- 2021
-
Abstract
- Summary Fruit ripening is a critical phase in the production and marketing of fruits. Previous studies have indicated that fruit ripening is a highly coordinated process, mainly regulated at the transcriptional level, in which transcription factors play essential roles. Thus, identifying key transcription factors regulating fruit ripening as well as their associated regulatory networks promises to contribute to a better understanding of fruit ripening. In this study, temporal gene expression analyses were performed to investigate banana fruit ripening with the aim to discern the global architecture of gene regulatory networks underlying fruit ripening. Eight time points were profiled covering dynamic changes of phenotypes, the associated physiology and levels of known ripening marker genes. Combining results from a weighted gene co‐expression network analysis (WGCNA) as well as cis‐motif analysis and supported by EMSA, Y1H, tobacco‐, banana‐transactivation experimental results, the regulatory network of banana fruit ripening was constructed, from which 25 transcription factors were identified as prime candidates to regulate the ripening process by modulating different ripening‐related pathways. Our study presents the first global view of the gene regulatory network involved in banana fruit ripening, which may provide the basis for a targeted manipulation of fruit ripening to attain higher banana and loss‐reduced banana commercialization.
- Subjects :
- 0106 biological sciences
0301 basic medicine
fruit ripening
Gene regulatory network
RNA-Seq
Plant Science
Computational biology
Biology
01 natural sciences
Critical phase
03 medical and health sciences
Gene Expression Regulation, Plant
transcriptional regulatory network
Gene expression
RNA‐Seq
Transcription factor
Gene
Research Articles
Plant Proteins
WGCNA
technology, industry, and agriculture
food and beverages
Musa
Ripening
banana
030104 developmental biology
Fruit
Agronomy and Crop Science
Research Article
Transcription Factors
010606 plant biology & botany
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology Journal
- Accession number :
- edsair.doi.dedup.....3b852050d05e5124d2cd5adeac7f0b46