A Method for Large-scale Separation of Amino-acids on Filter Paper

FOR quantitative determinations of amino-acids separated on filter paper, it is often desirable that the spots should contain a larger quantity of substance than is as a rule possible to achieve with unaffected resolution. A one-dimensional technique in which the substance to be analysed is applied along a line across the paper has been described by Yanofsky, Wasserman and Bonner1. I have successfully applied the following two-dimensional technique for separating comparatively large quantities of amino-acids in bacterial hydrolysates.