Back to Search
Start Over
Improving precise counting of mitotic cells in mantle cell lymphoma using phosphohistone H3 (PHH3) antibody
- Source :
- Journal of clinical pathology. 74(10)
- Publication Year :
- 2020
-
Abstract
- AimsMantle cell lymphoma (MCL) has a highly heterogeneous clinical course ranging from indolent, to aggressive and rapidly progressive disease. Proliferation is a strong predictor for disease outcome. In routine clinical practice, Ki-67 expression is used as a measure of proliferation. However, several studies have documented a high degree of inter-laboratory and inter-observer variation with Ki-67 immunohistochemistry. Phosphorylation of histone H3 occurs specifically during mitosis and hence serves as a specific marker for cells in mitosis.Methods and resultsWe investigated phosphohistone H3 (PHH3) immunohistochemistry as a proliferation maker in 28 tissue biopsies of MCL and compared the PHH3 results (as evaluated by direct microscopic visualisation and image analysis-aided scoring) with morphological subtyping, mitotic counts and Ki-67 index. We found PHH3-mitotic count was about sixfold higher than H&E-mitotic count (mitoses in 10 high power fields). Furthermore, PHH3-mitotic count in aggressive morphological variants of MCL was significantly higher than in usual MCL. The PHH3-mitotic count showed a strong linear correlation with PHH3-mitotic index (percentage positive cells).ConclusionsWe found PHH3 immunohistochemistry, a reliable mitosis-specific marker, in MCL. Performing precise counts and evaluating precise proliferation indices is easier with PHH3 immunohistochemistry. This contrasts with the conventional estimation of Ki-67 percentages by ‘eye-balling’.
- Subjects :
- 0301 basic medicine
Male
Mitosis
Cell Count
Lymphoma, Mantle-Cell
Biology
Pathology and Forensic Medicine
Histones
03 medical and health sciences
Histone H3
0302 clinical medicine
Predictive Value of Tests
medicine
Biomarkers, Tumor
Mitotic Index
Humans
Phosphorylation
Aged
Aged, 80 and over
Cell growth
Reproducibility of Results
General Medicine
Middle Aged
medicine.disease
Immunohistochemistry
Lymphoma
030104 developmental biology
Ki-67 Antigen
030220 oncology & carcinogenesis
biology.protein
Cancer research
Mantle cell lymphoma
Female
Antibody
Progressive disease
Subjects
Details
- ISSN :
- 14724146
- Volume :
- 74
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of clinical pathology
- Accession number :
- edsair.doi.dedup.....3b6d4864eb13e3024d118a96335b0c1e