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Proinflammatory Cytokines Regulate the Gene Expression of Hyaluronic Acid Synthetase in Cultured Rabbit Synovial Membrane Cells
- Source :
- Connective Tissue Research. 42:187-195
- Publication Year :
- 2001
- Publisher :
- Informa UK Limited, 2001.
-
Abstract
- To elucidate the mechanism of accumulation and fragmentation of hyaluronic acid (HA) under inflammatory conditions, we investigated the effect of proinflammatory cytokines on hyaluronic acid synthetase (HAS) mRNA expression using cultured rabbit synovial membrane cells. HASs mRNA levels were determined by real-time PCR. HAS2 mRNA expression was maximally enhanced 3.3- and 2.8-fold after 3-hour stimulation with IL-1beta (1 ng/ml) and after 1-hour stimulation with TNF-alpha (10 ng/ml). HAS3 mRNA expression was increased by a maximum of 4.3 times after 3-hour stimulation with IL-1beta (10 ng/ml), whereas 1-hour stimulation with TNF-alpha (10 ng/ml) and IFN-gamma (10 ng/ml) induced around a 2.5-fold increase in HAS3 mRNA. Although IFN-gamma (1-100 ng/ml) alone showed little effect on HAS2 mRNA expression, the effect was synergized by combined with both IL-1beta and TNF-alpha, substantially increasing HAS2 mRNA expression. These results suggest that proinflammatory cytokines regulate the HAS expression, and consequently may contribute to the accumulation and fragmentation of HA.
- Subjects :
- Stimulation
Xenopus Proteins
Biology
Biochemistry
Gene Expression Regulation, Enzymologic
Proinflammatory cytokine
Interferon-gamma
chemistry.chemical_compound
Rheumatology
Transferases
Hyaluronic acid
Gene expression
medicine
Animals
Drug Interactions
Orthopedics and Sports Medicine
RNA, Messenger
Glucuronosyltransferase
Hyaluronic Acid
Fragmentation (cell biology)
Molecular Biology
Cells, Cultured
Inflammation
Regulation of gene expression
Messenger RNA
Dose-Response Relationship, Drug
Tumor Necrosis Factor-alpha
Arthritis
Synovial Membrane
Glycosyltransferases
Membrane Proteins
Cell Biology
Molecular biology
Drug Combinations
medicine.anatomical_structure
chemistry
Cytokines
Rabbits
Synovial membrane
Hyaluronan Synthases
Interleukin-1
Subjects
Details
- ISSN :
- 16078438 and 03008207
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Connective Tissue Research
- Accession number :
- edsair.doi.dedup.....3b665ea57148a1ad07c17d9025565426