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Rat white matter injury model induced by endothelin-1 injection: technical modification and pathological evaluation
- Source :
- Scopus-Elsevier
- Publication Year :
- 2016
-
Abstract
- White matter injury is an important cause of functional disability of the brain. We comprehensively analyzed a modified endothelin-1 (ET-1) injection-induced white matter injury model in the rat which is very valuable for investigating the underlying mechanisms of subcortical ischemic stroke. ET-1 was stereotactically injected into the internal capsule of the rat. To avoid complications with leakage of ET-1 into the lateral ventricle, the safest trajectory angle to the target was established. Rats with white matter injury were extensively evaluated for structural changes and functional sequelae, using motor function tests, magnetic resonance (MR) imaging, histopathology evolution, volume estimation of the lesion, and neuroanatomical identification of affected neurons using the retrograde tracer hydroxystilbamidine. Optimization of the trajectory of the ET-1 injection needle provided excellent survival rate. MR imaging visualized the white matter injury 2 days after surgery. Motor function deficit appeared temporarily after the operation. Histological studies confirmed damage of axons and myelin sheaths followed by inflammatory reaction and gliosis similar to lacunar infarction, with lesion volume of less than 1% of the whole brain. Hydroxystilbamidine injected into the lesion revealed wide spatial distribution of the affected neuronal population. Compared with prior ET 1 injection models, this method induced standardized amount of white matter damage and temporary motor function deficit in a reproducible and safe manner. The present model is valuable for studying the pathophysiology of not only ischemia, but a broader set of white matter damage conditions in the lissencephalic brain.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Time Factors
Internal capsule
Stilbamidines
Ischemia
Poison control
Functional Laterality
Rats, Sprague-Dawley
White matter
Lesion
Amyloid beta-Protein Precursor
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
lacunar infarction
Internal Capsule
Leukoencephalopathies
Glial Fibrillary Acidic Protein
medicine
Animals
Swimming
Neurologic Examination
Hydroxystilbamidine
Endothelin-1
medicine.diagnostic_test
business.industry
rat model
General Neuroscience
Magnetic resonance imaging
General Medicine
Ectodysplasins
medicine.disease
Magnetic Resonance Imaging
Rats
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
chemistry
Gliosis
white matter injury
histopathology
retrograde tracing
medicine.symptom
business
Locomotion
Psychomotor Performance
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 16890035 and 00651400
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Acta Neurobiologiae Experimentalis
- Accession number :
- edsair.doi.dedup.....3b529f84b15cdca7687b6f9f0bb4af51