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Downregulation of surface sodium pumps by endocytosis during meiotic maturation of Xenopus laevis oocytes
- Source :
- The American journal of physiology. 258(1 Pt 1)
- Publication Year :
- 1990
-
Abstract
- During meiotic maturation, plasma membranes of Xenopus laevis oocytes completely lose the capacity to transport Na and K and to bind ouabain. To explore whether the downregulation might be due to an internalization of the sodium pump molecules, the intracellular binding of ouabain was determined. Selective permeabilization of the plasma membrane of mature oocytes (eggs) by digitonin almost failed to disclose ouabain binding sites. However, when the eggs were additionally treated with 0.02% sodium dodecyl sulfate (SDS) to permeabilize inner membranes, all sodium pumps present before maturation were recovered. Phosphorylation by [gamma-32P]ATP combined with SDS-polyacrylamide gel electrophoresis (PAGE) and autoradiography showed that sodium pumps were greatly reduced in isolated plasma membranes of eggs. According to sucrose gradient fractionation, maturation induced a shift of sodium pumps from the plasma membrane fraction to membranes of lower buoyant density with a protein composition different from that of the plasma membrane. Endocytosed sodium pumps identified on the sucrose gradient from [3H]ouabain bound to the cell surface before maturation could be phosphorylated with inorganic [32P]phosphate. The findings suggest that downregulation of sodium pumps during maturation is brought about by translocation of surface sodium pumps to an intracellular compartment, presumably endosomes. This contrasts the mechanism of downregulation of Na-dependent cotransport systems, the activities of which are reduced as a consequence of a maturation-induced depolarization of the membrane without a removal of the corresponding transporter from the plasma membrane.
- Subjects :
- Cell Membrane Permeability
Physiology
Sodium
chemistry.chemical_element
Ouabain
chemistry.chemical_compound
Xenopus laevis
Adenosine Triphosphate
medicine
Animals
Homeostasis
Sodium dodecyl sulfate
Ion transporter
Cell Membrane
Cell Biology
Membrane transport
Endocytosis
Kinetics
Meiosis
Membrane
Digitonin
chemistry
Biochemistry
Biophysics
Oocytes
Autoradiography
Female
Sodium-Potassium-Exchanging ATPase
Phosphorus Radioisotopes
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 00029513
- Volume :
- 258
- Issue :
- 1 Pt 1
- Database :
- OpenAIRE
- Journal :
- The American journal of physiology
- Accession number :
- edsair.doi.dedup.....3b4fc2b96e180ad95021bc10dbf2ca96