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Antibody titers against the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2 induced by BNT162b2 vaccination measured using automated chemiluminescent enzyme immunoassay
Antibody titers against the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2 induced by BNT162b2 vaccination measured using automated chemiluminescent enzyme immunoassay
- Source :
- Journal of Infection and Chemotherapy
- Publication Year :
- 2021
- Publisher :
- Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd., 2021.
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Abstract
- BackgroundLevels of 50% neutralizing titer (NT50) reflect a vaccine-induced humoral immunity after the vaccination against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Measurements of NT50 are difficult to implement in large quantities. A high-throughput laboratory test is expected for determining the level of herd immunity against SARS-CoV-2.MethodsWe analyzed samples from 168 Japanese healthcare workers who had completed two doses of the BNT162b2 vaccine. We analyzed immunoglobulin G (IgG) index values against spike protein (SP) using automated chemiluminescent enzyme immunoassay system AIA-CL and analyzed the background factors affecting antibody titer. SP IgG index was compared with 50% neutralization titers.ResultsThe median SP IgG index values of the subjects (mean age = 43 years; 75% female) were 0.1, 1.35, 60.80, and 97.35 before and at 2, 4, and 6 weeks after the first dose, respectively. At 4 and 6 weeks after the first dose, SP IgG titers were found to have positive correlation with NT50 titer (r=0.7535 in 4 weeks; r=0.4376 in 6 weeks). Proportions of the SP IgG index values against the Alpha, Beta, Gamma, and Delta variants compared with the original strain were 2.029, 0.544, 1.017, and 0.6096 respectively. Older age was associated with lower SP IgG titer index 6 weeks after the first dose.ConclusionsSP IgG index values were raised at 3 weeks after two doses of BNT162b2 vaccination and have positive correlation with NT50. SP IgG index values were lower in the older individuals and against Beta and Delta strain.
- Subjects :
- Microbiology (medical)
Adult
Male
COVID-19 Vaccines
AIA-CL
Alpha (ethology)
Antibodies, Viral
Immunoglobulin G
Neutralization
Immunoenzyme Techniques
Variant of concern
Medicine
Healthcare workers
Humans
Pharmacology (medical)
BNT162 Vaccine
Aged
biology
medicine.diagnostic_test
business.industry
SARS-CoV-2
50% neutralization titer
Vaccination
Antibody titer
COVID-19
Antibodies, Neutralizing
Titer
Infectious Diseases
Immunoassay
Humoral immunity
Immunology
biology.protein
Coronavirus disease-2019
Original Article
Female
business
Subjects
Details
- Language :
- English
- ISSN :
- 14377780 and 1341321X
- Database :
- OpenAIRE
- Journal :
- Journal of Infection and Chemotherapy
- Accession number :
- edsair.doi.dedup.....3b4d40b5ffff1df6b33b4c3736b1714e