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[Pharmacognosical study during 40 years]
[Pharmacognosical study during 40 years]
- Source :
- Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. 127(10)
- Publication Year :
- 2007
-
Abstract
- In order to quality control for medicinal plants such as Aconitum charmicaelii, Rhemannia glutinosa, Atractyrodes lancea, Pinellia ternata, Panax species and Gentiana scabra were clonally micropropagated by tip tissue culture and embryogenetic techniques. Monoclonal antibodies against the bioactive compounds contained in the crude drugs were prepared and set up the ELISA as a high sensitive and quick determination method. A newly developed eastern blotting methodology can stain typically the only antigen molecule and the related compounds having a same aglycone. Knockout extract can be prepared by using an immunoaffinity column conjugated with monoclonal antibody, and its importance has been discussed. Single chain fragment-variable gene against solamargine was cloned and transformed to a host plant, Solanum chasianum resulting in the increase of antigen molecule as 2.5 to 3 times. Three biosynthetic enzymes regarding marihuana compounds, THCA-, CBDA-, CBCA-synthases were isolated. THCAsynthase was cloned, over expressed and confirmed its characteristics including FAD combining enzyme, and finally determined the structure by X-ray analysis. The distribution of THCAsynthase was also investigated using its and GFP hybrid gene. We found new functions for saffron like improving learning and memory and LTP for blocking by ethanol. A folk medicine in Taiwan, Anoectochilus formosanus was propagated in vitro and investigated opening new pharmacological activities in lipid metabolism.
- Subjects :
- Pinellia ternata
Pharmacognosy
medicine.drug_class
Immunoblotting
Immunoglobulin Variable Region
Pharmaceutical Science
Enzyme-Linked Immunosorbent Assay
Monoclonal antibody
Chromatography, Affinity
chemistry.chemical_compound
Tissue culture
Antigen
medicine
Animals
Humans
Amino Acid Sequence
Cloning, Molecular
Pharmacology
Solamargine
Plants, Medicinal
Gentiana scabra
biology
Plant Extracts
Antibodies, Monoclonal
biology.organism_classification
Eastern blot
Aglycone
Biochemistry
chemistry
Biotechnology
Subjects
Details
- ISSN :
- 00316903
- Volume :
- 127
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
- Accession number :
- edsair.doi.dedup.....3b3d169d6c63556e39e1dc58add849dc