Additional file 4: of PCBP1 depletion promotes tumorigenesis through attenuation of p27Kip1 mRNA stability and translation

Figure S2. PCBP1 overexpression inhibits cell cycle progression. (A). Representative immunofluorescence staining of A2780 and DLD-1 cells with EdU and Hoechst in cells with endogenous PCBP1 knockdown (PCBP1 KD), GFP-PCBP1 overexpression (PCBP1) and the additional p27KD in PCBP1-overexpressing cells (PCBP1-p27KD). (B). Statistical analyses of EdU positive cells in A. The EdU positive cells were counted randomly in 3 views and analyzed. (mean ± SEM, n = 3 per group). (C). Representative flow cytometry analysis of A2780 and DLD-1 cells with PCBP1 overexpression after EDU staining. Ratios of EdU + GFP-positive cells to GFP positive cells or GFP-PCBP1-positive cells are shown in the right-upper quadrant of each diagram. (PPT 2149 kb)