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Evaluating changes to Ralstonia pickettii in high-purity water to guide selection of potential calibration materials for online water bioburden analyzers
- Source :
- Journal of Industrial Microbiology & Biotechnology
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- Online water bioburden analyzers (OWBAs) can provide real-time feedback on viable bacteria in high-purity water (HPW) systems for pharmaceutical manufacturers. To calibrate and validate OWBAs, which detect bacteria using scattered light and bacterial autofluorescence, standards are needed that mimic the characteristics of bacteria in HPW. To guide selection of potential standards, e.g., fluorescent microspheres, a relevant bacterial contaminant, Ralstonia pickettii, was characterized for size, count, viability, and autofluorescence after exposure for 24 h to HPW or a nutrient environment. The cells exposed to HPW showed smaller sizes, with lower counts and autofluorescence intensities, but similar spectral features. The cell characteristics are discussed in comparison with a set of fluorescent microspheres, considering factors relevant to OWBAs. These studies suggest that fluorescent microspheres should be relatively small (
- Subjects :
- Materials science
Bioengineering
Environmental Microbiology - Original Paper
Applied Microbiology and Biotechnology
Bioburden
03 medical and health sciences
Fluorescent microspheres
Calibration
High-purity water
030304 developmental biology
Ralstonia pickettii
0303 health sciences
Chromatography
biology
030306 microbiology
Water
biology.organism_classification
Autofluorescence
Viability
Online water bioburden analyzers
Scattered light
Water Microbiology
Biotechnology
Subjects
Details
- ISSN :
- 14765535 and 13675435
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of Industrial Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....3b31e040bc609b156be61f03b500d15a