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Primary structure and transcriptional regulation of rat pepsinogen C gene
- Source :
- Journal of Biological Chemistry. 264:10193-10199
- Publication Year :
- 1989
- Publisher :
- Elsevier BV, 1989.
-
Abstract
- The entire rat pepsinogen C gene has been isolated from a rat genomic library, using the rat pepsinogen C cDNA as a probe. Southern blot analysis showed that there exists at least two rat pepsinogen C genes. The nucleotide sequences of the coding regions and the 5'- and 3'-flanking regions of one of the rat pepsinogen C genes have been determined. This gene is split into 9 exons interrupted by eight intervening sequences. The 5'-flanking region is similar to that of the human pepsinogen C gene, but only the former has the core sequence of the Sp1 binding site. The amount of transcripts of the rat pepsinogen C genes was found to increase during development, and a similar increase was shown to be induced by injection of hydrocortisone. As a candidate of a factor which regulates the transcription, we found a 25-kDa protein by Southwestern blotting. It binds to a specific site in the 5'-flanking region of the gene only in the presence of Mg2+ ion, and it is present in the nuclear fraction of the gastric mucosa but not of the liver.
- Subjects :
- Transcription, Genetic
Pepsinogen C
Molecular Sequence Data
Restriction Mapping
Biology
digestive system
Biochemistry
Exon
Complementary DNA
Genes, Regulator
Animals
Coding region
Amino Acid Sequence
Southwestern blot
Molecular Biology
Gene
Southern blot
Base Sequence
Pepsinogens
Nucleic acid sequence
Nucleic Acid Hybridization
Rats, Inbred Strains
DNA
Cell Biology
Molecular biology
digestive system diseases
Rats
Blotting, Southern
Genes
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 264
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....3b2a2e131c4dae48062659499b217764