Propolis Standardized Extract (EPP-AF (R)), an Innovative Chemically and Biologically Reproducible Pharmaceutical Compound for Treating Wounds

Made available in DSpace on 2013-08-28T14:07:38Z (GMT). No. of bitstreams: 1 WOS000303805500008.pdf: 934790 bytes, checksum: 5129f2b95886d191ecd3dd83b9c749e6 (MD5) Made available in DSpace on 2013-09-30T19:23:17Z (GMT). No. of bitstreams: 2 WOS000303805500008.pdf: 934790 bytes, checksum: 5129f2b95886d191ecd3dd83b9c749e6 (MD5) WOS000303805500008.pdf.txt: 46028 bytes, checksum: c7220368485f95a3f2bf0d5b66f60ddb (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:34:08Z No. of bitstreams: 2 WOS000303805500008.pdf: 934790 bytes, checksum: 5129f2b95886d191ecd3dd83b9c749e6 (MD5) WOS000303805500008.pdf.txt: 46028 bytes, checksum: c7220368485f95a3f2bf0d5b66f60ddb (MD5) Made available in DSpace on 2014-05-20T15:34:08Z (GMT). No. of bitstreams: 2 WOS000303805500008.pdf: 934790 bytes, checksum: 5129f2b95886d191ecd3dd83b9c749e6 (MD5) WOS000303805500008.pdf.txt: 46028 bytes, checksum: c7220368485f95a3f2bf0d5b66f60ddb (MD5) Previous issue date: 2012-01-01 The aim of this study was to develop a formulation, containing the propolis standardized extract (EPP-AF (R)), which can assist in the healing of skin lesions. To achieve this objective the antimicrobial activity and chemical composition of the propolis extract was determined. The final product was subjected to in vitro and in vivo pre-clinical evaluation. The broth macrodi-lution method was used to determine the antimicrobial activity of the extracts and formulations against the microorganisms most commonly found in burns, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis. Wistar rats with puncture wounded skin were used to evaluate the wound healing properties of propolis. The results of chemical and biological characterization demonstrated the batch-to-batch reproducibility of the standardized extract which is an unprecedented result. The antimicrobial and wound healing activity of the pharmaceutical studied showed the best results when samples contain 3.6% propolis, suggesting that this is the most promising composition. Univ São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Lab Tecnol Farmaceut, BR-14043903 Ribeirao Preto, SP, Brazil Apis Flora Indl Coml Ltda, Lab Pesquisa Desenvolvimento & Inovacao, BR-14020670 Ribeirao Preto, SP, Brazil Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil